Euthanasia and Tissue Processing
Animals were euthanised 20 weeks after AAV surgery via transcardial
perfusion fixation under terminal pentobarbital anaesthesia (50 mg/kg).
Brains were post-fixed in 4% paraformaldehyde for 24 h before being
cryoprotected in a 30% sucrose solution with 0.1% sodium azide. Using
a freezing sledge microtome (Bright, Cambridgeshire, UK), 30μm serial
coronal sections were collected in a series of 6.