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Impact of Fluoride on Epigenetic and Metabolic Dynamics in the Ileum: Unveiling the Adaptive Responses in NOD Mice
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  • Marília Afonso Rabelo Buzalaf,
  • Aline Dionizio,
  • Aislan Quintiliano Delgado,
  • João Paulo Zanardini de Lara,
  • Ana Carolina Magalhães,
  • José Roberto Bosqueiro,
  • Juliana Sanches Trevizol,
  • Nathalia Rabelo Buzalaf
Marília Afonso Rabelo Buzalaf
Universidade de Sao Paulo Faculdade de Odontologia de Bauru

Corresponding Author:[email protected]

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Aline Dionizio
Universidade de Sao Paulo Faculdade de Odontologia de Bauru
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Aislan Quintiliano Delgado
Universidade Estadual Paulista Julio de Mesquita Filho - Campus de Bauru
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João Paulo Zanardini de Lara
Universidade de Sao Paulo Faculdade de Odontologia de Bauru
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Ana Carolina Magalhães
Universidade de Sao Paulo Faculdade de Odontologia de Bauru
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José Roberto Bosqueiro
Universidade Estadual Paulista Julio de Mesquita Filho - Campus de Bauru
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Juliana Sanches Trevizol
Universidade de Sao Paulo Faculdade de Odontologia de Bauru
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Nathalia Rabelo Buzalaf
Universidade de Sao Paulo Faculdade de Odontologia de Bauru
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Abstract

Fluoride (F) has been employed worldwide to control dental caries. More recently, it has been suggested that the consumption of low doses of F in the drinking water may reduce blood glucose levels, introducing a new perspective for the use of F for the management of blood glucose. However, the exact mechanism by which F affects blood glucose levels remains largely unexplored. Given that the small gut plays a pivotal role in glucose homeostasis, the aim of this present study was to investigate the proteomic changes induced by low doses of F in the ileum of female non-obese-diabetic (NOD) mice. Forty-two female NOD mice were divided into two groups based on the F concentration in their for 14 weeks: 0 (control) or 10 mg/L. At the end of the experimental period, the ileum was collected for proteomic and Western Blotting analyses. Proteomic analysis indicated an increase in isoforms of actin, gastrotropin (confirmed by Western Blotting), several H2B histones and enzymes involved in antioxidant processes, as well as a decrease in enzymes essential for energy metabolism. In summary, our data indicates an adaptive response of the organism to preserve protein synthesis in the ileum, despite significant alterations in energy metabolism typically induced by F, therefore highlighting the safety of controlled fluoridation in water supplies.
Submitted to Cell Biochemistry & Function
04 Feb 2024Editorial Decision: Revise Major
21 Feb 20241st Revision Received
21 Feb 2024Submission Checks Completed
21 Feb 2024Review(s) Completed, Editorial Evaluation Pending
21 Feb 2024Assigned to Editor
29 Feb 2024Editorial Decision: Accept