Functional annotation of the differential miRNAs
In order to analyze the biological function of differential miRNAs, the
predicted miRNAs target genes were annotated using gene ontology
enrichment (ftp://ftp.ncbi.nih.gov/gene/DATA/gene2go.gz) and Kyoto
Encyclopedia of genes and genomes pathway analysis
(http://www.genome.jp/kegg). The KEGG analysis provides information on
the signal transduction and disease pathway of target genes. This
information provides a basis for studying the function and involvement
of differential miRNAs in pathways. GO is an internationally
standardized gene function classification system. It includes three
ontologies that describe the molecular functions of genes, cellular
components and the biological processes involved.