Establishment of a mouse model of hepatic portal vein infection
The cysts were removed from the gerbils, and the protoscoleiae were isolated(Fig 1A). 1% eosin staining was used to identify the viability of protoscolmus, and the viability exceeded 90%, which can be used for infection. A mouse model of secondary infection was established by injecting the protoscolmus via the hepatic portal vein. B-ultrasound was used to detect the liver lesion of mice after 3 mouths of infection, as is shown in Fig 1B, there were obvious lesions in the liver tissue of the mice in the infection group, compared with the control group. The entry of pathogens into the body can induce the body to generate an immune response. So the E. multiloculari s -specific antibodies in the serum was detected by Enzyme-linked immunosorbent assay (ELISA). The results showed that the levels of E. multiloculari s-specific IgG, IgM, IgA and IgE antibodies in the serum of mice in the infection group increased significantly(Fig 1C-F). And the total IgG, IgM, IgA, and IgE antibodies in serum were also significantly increased(Fig 1G-J). This suggested that the mouse model of infection through the hepatic portal vein was successfully constructed and E. multilocularis infection can induce high levels of antibody responses.