Establishment of a mouse model of hepatic portal vein infection
The cysts were removed from the gerbils, and the protoscoleiae were
isolated(Fig 1A). 1% eosin staining was
used to identify the viability of protoscolmus, and the viability
exceeded 90%, which can be used for infection. A mouse model of
secondary infection was established by injecting the protoscolmus via
the hepatic portal vein. B-ultrasound was used to detect the liver
lesion of mice after 3 mouths of infection, as is shown in Fig 1B, there
were obvious lesions in the liver tissue of the mice in the infection
group, compared with the control group. The entry of pathogens into the
body can induce the body to generate an immune response. So the E.
multiloculari s -specific antibodies in the serum was detected by
Enzyme-linked immunosorbent assay (ELISA). The results showed that the
levels of E. multiloculari s-specific IgG, IgM, IgA and IgE
antibodies in the serum of mice in the infection group increased
significantly(Fig 1C-F). And the total IgG,
IgM, IgA, and IgE antibodies in serum were also significantly
increased(Fig 1G-J). This suggested that
the mouse model of infection through the hepatic portal vein was
successfully constructed and E. multilocularis infection can
induce high levels of antibody responses.