Construction of infection mouse model
The infection mouse model was established by injecting PSCs via the
hepatic portal vein. In briefly, The mice in infection group were
injected with PSCs via portal vein and portal saline injection was
defined as control group. A middle abdominal incision was made in mice
under anaesthetic and 2000 PSCs/200μl sediment or saline was injected
via portal vein by using a 0.45 × 15RWLB venous infusion needle. After
injection, a cotton bud was pressed on the puncture site for 5 min to
provide haemostasis and to prevent intraperitoneal spillage of the PSCs.
The abdominal cavity was then closed and then the mice were put on the
warm stage to promote waking.