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\begin{document}

\title{People with obesity exhibit losses in muscle proteostasis that are
partly improved by exercise training}



\author[1]{Kanchana Srisawat}%
\author[1]{Connor Stead}%
\author[1]{Katie Hesketh}%
\author[1]{Mark Pogson}%
\author[1]{Juliette Strauss}%
\author[1]{Matt Cocks}%
\author[1]{Ivo Siekmann}%
\author[2]{Stuart Phillips}%
\author[1]{Paulo Lisboa}%
\author[1]{Sam Shepherd}%
\author[1]{Jatin Burniston}%
\affil[1]{Liverpool John Moores University}%
\affil[2]{McMaster University}%


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  \date{June 17, 2024}


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\begin{abstract}
This pilot experiment examines if a loss in muscle proteostasis occurs
in people with obesity and whether endurance exercise positively
influences either the abundance profile or turnover rate of proteins in
this population. Men with (n = 3) or without (n = 4) obesity were
recruited and underwent a 14-d measurement protocol of daily deuterium
oxide (D2O) consumption and serial biopsies of vastus lateralis muscle.
Men with obesity then completed 10-weeks of high-intensity interval
training (HIIT), encompassing 3 sessions per week of cycle ergometer
exercise with 1 min intervals at 100 \% maximum aerobic power
interspersed by 1 min recovery periods. The number of intervals per
session progressed from 4 to 8, and during weeks 8-10 the 14-d
measurement protocol was repeated. Proteomic analysis detected 352
differences (p \textless{} 0.05, false discovery rate \textless{} 5\%)
in protein abundance and 19 (p \textless{} 0.05) differences in protein
turnover, including components of the ubiquitin-proteasome system. HIIT
altered the abundance of 53 proteins and increased the turnover rate of
22 proteins (p \textless{} 0.05) and tended to benefit proteostasis by
increasing muscle protein turnover rates. Obesity and insulin resistance
are associated with compromised muscle proteostasis, which may be
partially restored by endurance exercise.%
\end{abstract}%



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