Figure 4. (a) Integrated scanning curves of MANP-FLIA with different PCT concentrations in serum. (b) Calibration curves of MANP-FLIA with magnetic operation (red), MANP-FLIA without magnetic operation (black), and AuNPs-LFIA (blue) with different PCT concentrations in serum. (c) Fluorescent photographs of MANP-FLIA test strips in response to different concentrations of PCT in serum after magnetic enrichment and separation (0–250 ng mL−1). (d) SEM images of the T line region on the NC membrane after running with MANP6:6@mAbs at PCT concentrations of (i) 0 ng mL−1 and (ii) 100 ng mL−1. (e) Specificity evaluation of the proposed MANP-FLIA platform toward various inspected species. The concentration of PCT is 1 ng mL−1, and the concentration of all the other interfering species is 1000 ng mL−1. (f) Comparison of the proposed MANP-FLIA method with the standard clinical CLIA method in actual serum samples. (g) Correlation analysis between the proposed method and the standard CLIA method.