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Detection of biological loads in sewage using the automated robot-driven photoelectrochemical biosensing platform
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  • Yiming Zhang,
  • Zhi Chen,
  • Songrui Wei,
  • Yujun Zhang,
  • Hai Fu,
  • Han Zhang,
  • Defa Li,
  • Zhongjian Xie
Yiming Zhang
Shenzhen Children’s Hospital
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Zhi Chen
Shenzhen University
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Songrui Wei
Shenzhen University
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Yujun Zhang
Shenzhen Children’s Hospital
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Hai Fu
Shenzhen Children’s Hospital
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Han Zhang
Shenzhen University
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Defa Li
Shenzhen Children’s Hospital
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Zhongjian Xie
Shenzhen Children’s Hospital

Corresponding Author:[email protected]

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Abstract

Real-time polymerase chain reaction (RT-PCR) remains the most prevalent molecular detection technology for sewage analysis but is plagued with numerous disadvantages, such as time consumption, stringent equipment requirements, and susceptibility to false negatives. In this study, we construct an automated robot-driven photoelectrochemical biosensing platform that utilizes the CRISPR/Cas12a system, to achieve fast, ultrasensitive, high specificity detection of biological loads in sewage. The Shennong-1 robot integrates several functional modules, involving sewage sampling and pretreatment to streamline the sewage monitoring. A screen-printed electrode is employed with a vertical graphene-based working electrode and enhanced with surface-deposited Au nanoparticles (NPs). CdTe/ZnS quantum dots (QDs) are further fabricated through the double-stranded DNA anchored on Au nanoparticles. Using cDNA template of Omicron BA.5 spike gene as a model, the photoelectrochemical biosensor demonstrates excellent analytical performance, with a lower detection limit of 2.93×102 zM and an outstanding selectivity at the level of single-base mutation recognition. Furthermore, the rapid, accurate detection of BA.5 in sewage demonstrates the feasibility of the photoelectrochemical platform for sewage monitoring. In conclusion, this platform allows early detection and tracking of infectious disease outbreaks, providing timely data support for public health institutions to take appropriate prevention and control measures.
06 Sep 2023Submitted to Exploration
13 Sep 2023Submission Checks Completed
13 Sep 2023Assigned to Editor
05 Oct 2023Reviewer(s) Assigned
23 Oct 2023Review(s) Completed, Editorial Evaluation Pending
31 Oct 2023Editorial Decision: Revise Minor
14 Nov 20231st Revision Received
20 Nov 2023Submission Checks Completed
20 Nov 2023Assigned to Editor