Flow cytometry
The cells were stained with fluorescence-conjugated Abs against THBS1
(Cat # MA5-13398, Invitrogen/Thermo Fisher Scientific, Waltham, MA),
CD4, IFN‐γ, IL-17 or Foxp3 (eBioscience, San Diego, CA). For
intracellular staining of cytokines, the cells were treated with an
intracellular fixation buffer (eBioscience™ IC Fixation Buffer,
Invitrogen/Thermo Fisher Scientific) before staining. For Foxp3
staining, eBioscience™ Foxp3/Transcription Factor Staining Buffer Set
(Cat # 00-5523-00, Invitrogen/Thermo Fisher Scientific) was used. The
stained cells were assayed using a flow cytometer (Cytek Aurora Flow
Cytometer, Cytek biosciences) and data were analyzed using FlowJo
software (Tree Star, Inc., Ashland, OR).