Figure 1. Fut1 KO splenocytes exhibit decreased levels of THBS1 in comparison to WT splenocytes.
A. Experimental protocol. Splenocytes were isolated from WT orFut1 KO mice and subsequently subjected to assays. Scatter plot from RNA sequencing. The plot displays the normalized data in a logarithmic scale for each gene, comparing WT splenocytes on the x-axis to Fut1 KO splenocytes on the y-axis.
B. Volcano plot comparison of gene expression betweenFut1 KO and WT splenocytes. The x-axis indicates the fold change in a logarithmic scale, while the y-axis indicates the statistical significance of the difference in expression (p value from at test). Red dots represent 69 transcripts that were upregulated (> 2-fold, p- value < 0.05), while green dots represent 55 transcripts that were downregulated in Fut1 KO splenocytes compared to WT splenocytes.
C. Bar chart comparison of gene expression based on gene ontology categories. The y axis represents the percentage of DEGs out of the total genes in each category. The number displayed above each bar represents the count of genes upregulated (red bar) or downregulated (green bar) in the specific category.
D, E. Heat maps of RNA sequencing data of Fut1 KO splenocytes compared to WT splenocytes. The heat map in D represents the complete list of DEGs, while the heat map in E presents the DEGs within the gene ontology category of immune or inflammatory response.Thbs1 exhibited the most pronounced downregulation in Fut1KO splenocytes when compared to WT splenocytes.
F. qRT-PCR for Thbs1 in splenocytes or corneal tissues collected from WT and Fut1 KO mice. The mRNA levels are presented as fold changes relative to the levels in the WT control group.
G. Representative and quantitative flow cytometry results for THBS1+ cells in WT or Fut1 KO splenocytes.
***p < 0.001, ****p < 0.0001, ns: not significant, as analyzed by one-way ANOVA with Tukey’s test (F) or Student’st -test (G)