Figure 2. Fut1 KO splenocytes enhance T cell
proliferation and differentiation, while replenishing THBS1 reverses
these effects.
A. Experimental design. CD4+ cells isolated
from WT mice were cocultured with either splenocytes isolated from WT
mice or splenocytes from Fut1 KO mice for 5 d, after which the
cells were subjected to further assays. In some cocultures, 5 µg/mL
recombinant THBS-1 was added.
B. CFSE dilution assay for CD4+ cell
proliferation
C, D. Representative and quantitative flow cytometry results
for IFN-γ+CD4+ and
IL-17+CD4+ cells (C) and for
Foxp3+CD4+ cells (D)
E. ELISA for secreted levels of IL-2, IFN-γ, IL-17 and IL-10 in
the cocultures of CD4+ cells and splenocytes.
*p < 0.05, **p < 0.01, ***p < 0.001, ****p
< 0.0001, ns: not significant, as analyzed by one-way ANOVA
with Tukey’s test or Kruskal-Wallis test with Dunn’s
multiple-comparisons test (D)