Figure 1. Fut1 KO splenocytes exhibit decreased levels of
THBS1 in comparison to WT splenocytes.
A. Experimental protocol. Splenocytes were isolated from WT orFut1 KO mice and subsequently subjected to assays. Scatter plot
from RNA sequencing. The plot displays the normalized data in a
logarithmic scale for each gene, comparing WT splenocytes on the x-axis
to Fut1 KO splenocytes on the y-axis.
B. Volcano plot comparison of gene expression betweenFut1 KO and WT splenocytes. The x-axis indicates the fold change
in a logarithmic scale, while the y-axis indicates the statistical
significance of the difference in expression (p value from at test). Red dots represent 69 transcripts that were upregulated
(> 2-fold, p- value < 0.05), while green
dots represent 55 transcripts that were downregulated in Fut1 KO
splenocytes compared to WT splenocytes.
C. Bar chart comparison of gene expression based on gene
ontology categories. The y axis represents the percentage of DEGs out of
the total genes in each category. The number displayed above each bar
represents the count of genes upregulated (red bar) or downregulated
(green bar) in the specific category.
D, E. Heat maps of RNA sequencing data of Fut1 KO
splenocytes compared to WT splenocytes. The heat map in D represents the
complete list of DEGs, while the heat map in E presents the DEGs within
the gene ontology category of immune or inflammatory response.Thbs1 exhibited the most pronounced downregulation in Fut1KO splenocytes when compared to WT splenocytes.
F. qRT-PCR for Thbs1 in splenocytes or corneal tissues
collected from WT and Fut1 KO mice. The mRNA levels are presented
as fold changes relative to the levels in the WT control group.
G. Representative and quantitative flow cytometry results for
THBS1+ cells in WT or Fut1 KO splenocytes.
***p < 0.001, ****p < 0.0001, ns: not significant,
as analyzed by one-way ANOVA with Tukey’s test (F) or Student’st -test (G)