Statistical analysis
Statistical analysis was performed using Prism software (GraphPad, San Diego, CA). The normal distribution of data in each group was assessed using the Shapiro-Wilk test and the Kolmogorov-Smirnov test. For comparisons between more than two groups, one-way ANOVA with Tukey’s test or Kruskal-Wallis test with Dunn’s multiple-comparisons test was employed to determine significant differences in mean values. Student’st -test was used for comparisons between two groups. The data were presented as mean ± SD. Statistical significance was defined as p< 0.05.