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Comparative proteome analysis of retinal hypoxia--ischemia in acute ocular hypertension model with TMT-based quantitative proteomics
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  • Guangyi Huang,
  • Yunru Lin,
  • Fen Tang,
  • Hui Huang,
  • Qi Chen,
  • Ning Su,
  • Ling Cui,
  • Fan Xu,
  • Chaolan Shen
Guangyi Huang
People's Hospital of Guangxi Zhuang Autonomous Region
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Yunru Lin
People's Hospital of Guangxi Zhuang Autonomous Region
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Fen Tang
People's Hospital of Guangxi Zhuang Autonomous Region
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Hui Huang
People's Hospital of Guangxi Zhuang Autonomous Region
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Qi Chen
People's Hospital of Guangxi Zhuang Autonomous Region
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Ning Su
People's Hospital of Guangxi Zhuang Autonomous Region
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Ling Cui
People's Hospital of Guangxi Zhuang Autonomous Region
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Fan Xu
People's Hospital of Guangxi Zhuang Autonomous Region
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Chaolan Shen
People's Hospital of Guangxi Zhuang Autonomous Region

Corresponding Author:[email protected]

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Abstract

Acute glaucoma’s main sign is acute ocular hypertension (AOH), leading to retinal ganglion cell (RGC) death and irreversible visual loss. However, there is currently no approved effective therapy for this condition. This research aimed to identify the major regulators and the overall protein changes involved in AOH-induced RGC death. Mass-spectrometry was used to analyze proteomic patterns in the retinal protein extracts from the AOH and sham-group, and then Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway studies were performed. In proteomics analysis, we identified 92 proteins in the AOH group compared to the control group, with 58 proteins being up-regulated and 34 proteins being down-regulated. Western blot and biochemical assay analyses identified changes in Fatty acid-binding protein 7 (FABP7), and caveolin-1(Cav-1) that were related to fatty acid metabolism and ocular inflammatory signaling. Moreover, variations in the expression of the proteins Galectin-1 (Gal-1), S100 calcium-binding protein A6 (S100a6), and Visinin-like protein-1 (VILIP) was shown, all of which were associated to the process of neuronal ischemia. Our investigation demonstrated that neuroinflammation and fatty acid metabolism were involved in retinal impairment following AOH, offering a potential therapeutic strategy for acute glaucoma.
09 Aug 2023Submitted to Clinical Applications
30 Aug 2023Submission Checks Completed
30 Aug 2023Assigned to Editor
30 Aug 2023Review(s) Completed, Editorial Evaluation Pending
01 Sep 2023Reviewer(s) Assigned