2.3.3 Vibratome sectioning
Fixed leaf samples were washed twice in PBS and embedded in 8% agarose.
The agarose blocks were sectioned using a vibratome (VT1000 S, Leica) to
obtain 150 µm-thick sections. These specimens were mounted in 8:2
glycerol:PBS on a microscope slide and imaged with a wide-field
microscope (ECLIPSE Ni-U, Nikon) fitted with a digital camera.