The Second Generation µSI Process Analyzer System: The A2P2
System
To minimize the instrument’s footprint, the µSI process analyzer system
was integrated into a commercially available Agilent 1290 Infinity II
Ultra-High-Performance Liquid Chromatography (UHPLC) system and an
optional auxiliary mass spectrometer. This system enables both peptide
mapping and glycosylation assays and can function as a standalone sample
purification platform, complement the Tecan platform, or be used for
subsequent product quality assays like aggregation and charge variants.
This new system is referred to as the A2P2 system (Figure 3 ).
Innovative designs enable user-selectable sample preparation protocols
to be executed on a platformed fluidic design, without physically
reconfiguring flow paths inside the A2P2 system. The design transforms a
typical UHPLC system into an A2P2-enabled LC system while maintaining
its original functionalities as a standalone LC system.
The A2P2 methodology enables the integration of modality agnostic
analytics with automatic sample acquisition, preparation, and analysis
in a compact form factor, resulting in a more than 50% reduction in
footprint while providing enhanced functionalities. Additionally, the
A2P2 methodology can be used for online, at-line, or off-line
applications, enabling both on-the-floor and laboratory implementations.