The Second Generation µSI Process Analyzer System: The A2P2 System
To minimize the instrument’s footprint, the µSI process analyzer system was integrated into a commercially available Agilent 1290 Infinity II Ultra-High-Performance Liquid Chromatography (UHPLC) system and an optional auxiliary mass spectrometer. This system enables both peptide mapping and glycosylation assays and can function as a standalone sample purification platform, complement the Tecan platform, or be used for subsequent product quality assays like aggregation and charge variants. This new system is referred to as the A2P2 system (Figure 3 ).
Innovative designs enable user-selectable sample preparation protocols to be executed on a platformed fluidic design, without physically reconfiguring flow paths inside the A2P2 system. The design transforms a typical UHPLC system into an A2P2-enabled LC system while maintaining its original functionalities as a standalone LC system.
The A2P2 methodology enables the integration of modality agnostic analytics with automatic sample acquisition, preparation, and analysis in a compact form factor, resulting in a more than 50% reduction in footprint while providing enhanced functionalities. Additionally, the A2P2 methodology can be used for online, at-line, or off-line applications, enabling both on-the-floor and laboratory implementations.