Sampling |
Aspirate 2 mg protein sample from source |
Affinity Cleanup |
Protein retained, purified, and eluted from the
affinity column and deposit into the holding coil downstream of the
affinity column. |
Denaturation |
Deliver the purified protein from the holding coil into
the 30C incubator. Add denaturation solution. Incubate for 10
minutes. |
Reduction |
Add reduction solution into the 30C incubator. Incubate for
30 minutes |
Alkylation |
Add alkylation solution into the 30C incubator. Incubate
for 20 minutes. |
Quenching |
Add quenching solution. Incubate for 5
minutes. |
Desalting |
Transfer the entire amount of mixture from the 30C incubator
to the desalting column. Capture the desalted protein through the 3-way
valve to the 40C incubator. |
Enzymatic Digestion |
Add enzyme solution into the 40C incubator.
Incubate for 10 minutes. |
LC Injection |
Deliver the digested protein mixture to the LC sample
loop and initiate LC-MS method. |