Process Step Action
Sampling Aspirate 2 mg protein sample from source
Affinity Cleanup Protein retained, purified, and eluted from the affinity column and deposit into the holding coil downstream of the affinity column.
Denaturation Deliver the purified protein from the holding coil into the 30C incubator. Add denaturation solution. Incubate for 10 minutes.
Reduction Add reduction solution into the 30C incubator. Incubate for 30 minutes
Alkylation Add alkylation solution into the 30C incubator. Incubate for 20 minutes.
Quenching Add quenching solution. Incubate for 5 minutes.
Desalting Transfer the entire amount of mixture from the 30C incubator to the desalting column. Capture the desalted protein through the 3-way valve to the 40C incubator.
Enzymatic Digestion Add enzyme solution into the 40C incubator. Incubate for 10 minutes.
LC Injection Deliver the digested protein mixture to the LC sample loop and initiate LC-MS method.