The Flow-Thru Vial (FTV) in the A2P2 system
The FTV enables all sample processing functions in the A2P2 system and directs processed intermediate and/or fully processed samples to return from the sample preparation unit to the autosampler for further processing and/or chromatographic injection. The FTV can also act as a sample receptacle connected to the bioreactor or process stream, enabling online sample processing. The design and fabrication of the FTV were carried out in-house to ensure proper testing and quick turnaround time for prototyping. As shown in Figure 4 , a threaded hole was used to connect the vial to the system, and an angled gutter at the top of the vial allows liquid to flow directly to waste. As liquid flows into the bottom of the vial, it forces excess liquid into the gutter, where it can gravity drain to waste. Because the vial has unique features, a vial holder was also fabricated in-house to ensure proper vial alignment. The FTV was made from polyether ether ketone (PEEK) for its chemical inertness, and the vial holder was made from aluminum.