µSI Process Analyzer System for MAM Sample Prep
The detailed µSI process analyzer fluidic design for MAM sample
preparation is described in Figure 1 . It consists of a series
of columns, reaction chambers, tubing, liquid redirect valves, a
multi-position solvent selector valve, and a syringe pump. Software
scripting capabilities enable the µSI process analyzer system to
robustly execute the process steps described in Table 1 .
Test samples can be acquired directly from the bioreactor supernatant or
purified material. The sample is aspirated into the system through the
solvent selector valve and passed through an affinity purification
column where the protein of interest is bound, and the sample matrix is
removed as column flow-through. The purified protein is then eluted and
delivered to a thermostatic incubator where denaturation, reduction, and
alkylation reactions occur. This chemically processed sample undergoes
buffer exchange and is enzymatically digested in a second thermostatic
incubator before chromatographic separation and mass detection.