Real-time Glycosylation Data
Figure 7a displays a typical glycan map generated using the HILIC UPLC integrated µSI process analyzer system from preliminary proof-of-concept experiments; the results were comparable to those obtained off-line using the manual approach. Subsequently,Figure 7b verifies the integrated system’s performance over a 32-day period, using daily processing of the reference standard, indicating good system repeatability and robustness. Figure 7cshowcases results from online measurements over a 32-day period from a production-scale bioreactor. Each data point represents one bioreactor sample acquired daily online, each sample is bracketed by two reference standards to ensure assay acceptance and accuracy. This data provides insights that can guide process development and aid in manufacturing consistent, high-quality products.