Multi-Attribute Assay Sample Preparation in the µSI Process
Analyzer System
The multi-attribute method (MAM) assay utilizes peptide mapping
methodology to identify and quantify critical quality attributes (CQAs).
It is typically performed off-line through manual sample handling,
followed by Liquid Chromatography-Mass Spectroscopy (LC-MS) operation,
and generating results can take several days. Transitioning the
off-line, manual MAM assay into a real-time online LC-MS integrated µSI
process analyzer system, where results can be obtained within 2 to 3
hours, can guide process development activities, and provide valuable
insights into the performance of a biomanufacturing process.