2. Results and Discussion
C8-TPE-C4TAB-enhanced CL of luminol-Co2+-H2O2system. As a starting point for our study, we synthesized fluorescent surfactant C8-TPE-C4TAB according to our previously reported method and characterized it with1H NMR, MS, steady-state and time-resolved fluorescence spectrometry.34 All results revealed the successful preparation of C8-TPE-C4TAB (Figures S1-S3). As is known, surfactants could facilitate the CL reaction and enhance the CL signals.35,36 In this study, the CL signals of luminol-Co2+-H2O2 in the absence and presence of C8-TPE-C4TAB were recorded under pH 7.4 conditions. As shown in Figure 2, the CL intensity was dramatically enhanced (~20 folds) after the addition of 80 μM C8-TPE-C4TAB, indicating the feasibility of C8-TPE-C4TAB-improved CL intensity of luminol-Co2+-H2O2system. In addition, the reaction kinetics was also dramatically decelerated in the presence of C8-TPE-C4TAB. The enhanced intensity and extended CL emission kinetics indicated the possibility to construct long-lasting luminol-Co2+-H2O2system with the assistance of C8-TPE-C4TAB.