2.4 Determining growth
Strains were cultured in 5 ml LB medium at 37 oC and
180 rpm overnight, then 2 µl broth of different strains were used for
inoculating 200 µl fresh LB medium, or LB medium added with different
concentrations of glucose (0, 2, 5, 10 and 20 g/L) in 96-well
microplates. The microplates were cultured at 37 oC
and 180 rpm, and the growth curve of strains were recorded with an
automatic growth curve analyzer (Bioscreen Cpro, Finland).