Transcriptome sequencing
technologies
Transcriptome sequencing technology is a computational method that
determines the identity and abundance of RNA sequences in a biological
sample. This technology has been widely used to study dynamic gene
expression in diverse human tissues, including human bladder cancer. The
general workflow of RNA-Seq begins with isolation of RNAs from tissue
samples, library preparation, sequencing and computational analysis
(Figure 1 ). Below we summarize and discuss RNA-Seq techniques,
narrowing down to its application in human bladder cancer studies.