4.0 Review of RNA-Seq studies on bladder cancer
RNA sequencing has emerged as a powerful next-generation sequencing
(NGS) technology tool for unbiased identification of gene expression,
discovery and quantification of novel transcripts, and identification of
alternatively spliced genes.22,41 Consequently, recent
progress in RNA-Seq has broadened the understanding of the molecular
pathogenesis of cancers, including bladder cancer. RNA-Seq has been
successfully applied in bladder cancer research for earlier detection,
establishing pathological origin, and defining the aberrant genes and
dysregulated molecular pathways across patient groups. Thus far, eight
studies have been reported on bladder cancer that made use of the
RNA-Seq in understanding the bladder cancer pathology. These studies are
summarised based on sample, bladder cancer grades and library
preparation techniques employed (Table 2 ).