Transcriptome sequencing technologies

Transcriptome sequencing technology is a computational method that determines the identity and abundance of RNA sequences in a biological sample. This technology has been widely used to study dynamic gene expression in diverse human tissues, including human bladder cancer. The general workflow of RNA-Seq begins with isolation of RNAs from tissue samples, library preparation, sequencing and computational analysis (Figure 1 ). Below we summarize and discuss RNA-Seq techniques, narrowing down to its application in human bladder cancer studies.