3.5 Dysregulated glucose metabolism in adult F2 male and F2 female mice
We measured blood glucose at three lifetime points (8, 25, and 38 weeks) within the F2 exposure groups to understand the effects of grandmaternal exposure on adult F2 diabetic phenotypes. The F0 exposure of 245 ppb causes a T2D phenotype in F2 female mice starting at an early age (3 weeks) that is persistent through adulthood (38 weeks) (Figure 7.A ). In addition, F2 male mice developed dysregulated glucose metabolism only in late life (38 weeks) (Figure 7.B ). We identified no significant changes in AUC analysis (Supplemental Figure 4.A and 4.B ). These results indicate primordial germ cells are damaged by grandmaternal iAs exposure and result in adult metabolic disruption that is dose-specific.