3.2 F1 Females, but not males, show delayed glucose response
similar to type II diabetes
Blood glucose was measured at three lifetime points (8, 25, and 38
weeks) to understand if in utero iAs exposure causes type II
diabetes (T2D) associated phenotypes in F1 offspring. Throughout the
study, F1 male mice exhibited no differences in glucose metabolism
(Figure 4.B) . F1 female offspring of 10 ppb dams exhibited
consistent (i.e. multi-timepoint) dysregulated metabolism of glucose
during the 25- and 38-week oral glucose challenges (Figure
4.A). No significant differences were identified in Area Under the
Curve (AUC) analysis (Supplemental 1.A and 1.B). Our data
indicate a low exposure of 10 ppb iAs during pregnancy contributes to
irregular metabolism of glucose in late life F1 females.
3.3 Maternal iAs exposure does not influence anxiety or memory
behaviors in F1 or F2 offspring
We performed marble burying to determine whether gestational iAs
exposure induced anxiety and a Y-maze assessment for spatial memory
within adult F1 and F2 mice (Supplemental Figure 2,
Supplemental Figure 3 ). We identified no significant differences
between exposure groups and control for either generation in both the
marble burying and spontaneous alteration behavior in the Y-maze. These
results suggest that in utero iAs exposure does not cause anxiety or
impair spatial memory in F1 and F2 adult mice.