Glucose Tolerance Testing
At ages 8, 25, and 38 weeks, mice (n=4 per treatment) underwent a 6 hour fast before receiving a 100 μL glucose bolus by oral gavage. Blood glucose was monitored before glucose administration (0 minutes) and recorded every 15 minutes for 1.5 hours by Accu-Check Blood Glucose Meter (Roche Diabetes Care, Inc.). Statistical significance was determined by a 2-way ANOVA (p <0.05), and Area Under the Curve (AUC) was calculated using the AUC function in Prism 9.