Glucose Tolerance Testing
At ages 8, 25, and 38 weeks, mice (n=4 per treatment) underwent a 6 hour
fast before receiving a 100 μL glucose bolus by oral gavage. Blood
glucose was monitored before glucose administration (0 minutes) and
recorded every 15 minutes for 1.5 hours by Accu-Check Blood Glucose
Meter (Roche Diabetes Care, Inc.). Statistical significance was
determined by a 2-way ANOVA (p <0.05), and Area Under the
Curve (AUC) was calculated using the AUC function in Prism 9.