2.2 DNA extraction
Nucleic acid extraction was performed using two commercial kits: MagMAX™ Pathogen RNA/DNA Kit (ThermoFisher) and Nanobind CBB Big DNA Kit (Circulomics). 200 microliters (µl) of whole blood or homogenized tissue sample were extracted following the manufacturer’s instructions. The extracted DNA from the MagMax kit was sequenced on the Illumina MiSeq or Oxford Nanopore Technologies GridION platforms, while the high molecular weight DNA extracted with Circulomics kit was sequenced on the Nanopore PromethION platform.