2.1. Cell Culture-GBM Cell Lines
There are multiple distinct subpopulations that have been shown to respond differently to radiation and treatment with TMZ (Lee, 2016). Three GBM cell lines were employed in order to compare how different populations of cells respond to the treatment in GBM organoids. GBM cell lines U-87 MG (ATCC® HTB-14TM), A172 (ATCC® CRL-1620TM) (obtained from ATCC, Manassas, VA), and BT169 (ATCC®CRL-3413 ) were employed. Each cell line was cultured separately. U-87 MG and A172 cancer cell lines were cultured in high glucose Dulbecco’s Modified Eagle Medium (DMEM) with 10% fetal bovine serum (FBS), 1% L-glutamine, and 1% penicillin/streptomycin in a tissue culture incubator at 37°C with 5% CO2. B169 cell line was cultured in NeuroCult Basal Medium with proliferation Supplement, 20 ng/mL EGF, 20 ng/mL β-FGF and 2 µg/mL heparin sulfate in a tissue culture incubator at 37°C with 5% CO2.