Figure 4. LIVE/DEAD images of three tested cell lines with different combinations of αCT1 (0 and 100 μM) and TMZ (0, 10, 100, 1000 μM). A, LIVE/DEAD images of A172. B , LIVE/DEAD images of U87.C , LIVE/DEAD images of BT169. Green – Calcein AM-stained viable cells; Red – Ethidium homodimer-1-stained dead cell nuclei. Scale bar – 250 μm. LIVE/DEAD images of samples treated with 1 and 10 μM αCT1 can be found in Supplemental Figures 4-6.