2.1. Cell Culture-GBM Cell Lines
There are multiple distinct subpopulations that have been shown to
respond differently to radiation and treatment with TMZ (Lee, 2016).
Three GBM cell lines were employed in order to compare how different
populations of cells respond to the treatment in GBM organoids. GBM cell
lines U-87 MG (ATCC® HTB-14TM), A172
(ATCC® CRL-1620TM) (obtained from
ATCC, Manassas, VA), and BT169 (ATCC®CRL-3413™ ) were employed. Each cell line was
cultured separately. U-87 MG and A172 cancer cell lines were cultured in
high glucose Dulbecco’s Modified Eagle Medium (DMEM) with 10% fetal
bovine serum (FBS), 1% L-glutamine, and 1% penicillin/streptomycin in
a tissue culture incubator at 37°C with 5% CO2. B169
cell line was cultured in NeuroCult Basal Medium with proliferation
Supplement, 20 ng/mL EGF, 20 ng/mL β-FGF and 2 µg/mL heparin sulfate in
a tissue culture incubator at 37°C with 5% CO2.