2.6. αCT1 Peptide-Cx43 Interaction
αCT1 peptide conjugated with Fluorescein isothiocyanate (FITC- αCT1;
LifeTein) was used to visualize the distribution of αCT1 peptide inside
the cells during treatment. Four experimental groups were used: control
(no drug or peptide), TMZ (100 μM), FITC-αCT1 (100 μM), and combination
of 100 μM TMZ and 100 μM FITC-αCT1. Treatment was added 24 hours after
seeding the organoids and replenished on day 4, the organoids were fixed
with 4% PFA for 1 hour on day 7. Constructs were stained as described
in section 2.7 for Cx43.