Figure 6. ATP assay data and LIVE/DEAD images of patient derived
GBM cells. A, patient derived GBM cell viability after treatment with
TMZ at 0, 10, 100, or 1000 μM alone or in combination with αCT1 at
concentrations of 0, 1, 10, 100 μM. B , patient derived GBM cell
ATP data responding to TMZ at 1000 μM alone or in combination with αCT1
at concentrations of 0, 1, 10, 100 μM. C , LIVE/DEAD images of
patient derived cells after the treatment. Green – Calcein AM-stained
viable cells; Red – Ethidium homodimer-1-stained dead cell nuclei.
Treatment with lower concentrations of αCT1 (1 and 10 µM) can be found
in Supplemental Figure 7. Scale bar – 250 μm.