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Chemokine Receptors Expression on Peripheral CD4-Lymphocytes in Rheumatoid Arthritis: CD4+CD183+ As A Diagnostics Marker for Disease Activity in Rheumatoid Arthritis
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  • Samuel Asamoah Sakyi,
  • Tonnies Abeku Buckman,
  • Kwame Yeboah-Mensah,
  • Daniel Antwi-Berko,
  • Alfred Effah,
  • Ebenezer Senu,
  • Dzifa Dey,
  • Maxwell Antwi,
  • Joseph Yorke,
  • Andy Boateng,
  • Akwasi Addei,
  • Muniru Tanko,
  • Richard Boateng
Samuel Asamoah Sakyi
Kwame Nkrumah University of Science and Technology
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Tonnies Abeku Buckman
Kwame Nkrumah University of Science and Technology
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Kwame Yeboah-Mensah
Kwame Nkrumah University of Science and Technology
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Daniel Antwi-Berko
VU University Medical Centre Amsterdam
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Alfred Effah
Kwame Nkrumah University of Science and Technology
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Ebenezer Senu
Kwame Nkrumah University of Science and Technology

Corresponding Author:[email protected]

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Dzifa Dey
Korle Bu Teaching Hospital
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Maxwell Antwi
Koforidua Technical University
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Joseph Yorke
Kwame Nkrumah University of Science and Technology
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Andy Boateng
Kwame Nkrumah University of Science and Technology
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Akwasi Addei
Kwame Nkrumah University of Science and Technology
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Muniru Tanko
University for Development Studies
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Richard Boateng
Komfo Anokye Teaching Hospital
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Abstract

Background: T cell chemokines and its receptors play important roles in the development and progression of rheumatoid arthritis (RA). Their involvement has been reported in inflammatory autoimmune diseases. However, their role in RA in the Ghanaian population has not been explored. We evaluated the intracytoplasmic CD4+ T cell chemokine receptors in rheumatoid arthritis patients in Ghana and determined their relationship with disease activity. Methods. This case control study included 48 newly diagnosed RA patients and 30 apparent healthy controls from the orthopaedic units of Komfo Anokye Teaching Hospital (KATH), Kumasi and Korle-Bu Teaching Hospital (KBTH), Accra, Ghana. A well-structured questionnaire was used to obtain sociodemographic data. Blood samples were also collected and processed for flow cytometric analysis. Statistical analyses were done using SPSS version 26.0 and R programming language. Results: This study found a significant difference in age group (p < 0.0001), marital status (p ¬= 0.0210), occupation (p = 0.0140), educational level (p = 0.0210) and religion (p = 0.0100) between RA patients and healthy controls. Moreover, haemoglobin level (p = 0.0010), waist circumference (p < 0.0001) and hip circumference (p = 0.0040) were significantly different between RA patients and healthy controls. RA patients had significantly lower levels of CD4+CD183+ compared to the control group (p < 0.001), and was positively correlated with DAS score (r = 0.0397, p = 0.789). In Receiver Operator Characteristics analysis, CD4+CD183+ could significantly detect rheumatoid arthritis with a very high area under the curve (AUC = 0.687, p = 0.018). At a cut-off of 0.082, CD4+CD183+ was the best chemokine receptor for detecting RA with a sensitivity of 90.0%, specificity of 25.9%, a positive predictive value of 69.2%, and a negative predictive value of 58.3%. Conclusion: CD4+CD183+ could serve as useful diagnostics and disease-monitoring marker for rheumatoid arthritis in the Ghanaian population.
26 Feb 2023Submitted to Immunity, Inflammation and Disease
27 Feb 2023Submission Checks Completed
27 Feb 2023Assigned to Editor
01 Mar 2023Review(s) Completed, Editorial Evaluation Pending
14 Mar 2023Reviewer(s) Assigned
03 Apr 2023Editorial Decision: Revise Major
19 Apr 20231st Revision Received
24 Apr 2023Assigned to Editor
24 Apr 2023Submission Checks Completed
24 Apr 2023Review(s) Completed, Editorial Evaluation Pending
24 Apr 2023Reviewer(s) Assigned
10 May 2023Editorial Decision: Revise Major
15 May 20232nd Revision Received
16 May 2023Review(s) Completed, Editorial Evaluation Pending
16 May 2023Submission Checks Completed
16 May 2023Assigned to Editor
16 May 2023Reviewer(s) Assigned
25 Jul 2023Editorial Decision: Accept
Aug 2023Published in Immunity, Inflammation and Disease volume 11 issue 8. https://doi.org/10.1002/iid3.976