Figure 4 MORC2 mutants affect cortical neuron survival and neurite outgrowth.
A. Confocal images of eGFP cortical neuron at day 5 revealing soma and neurites of neurons expressing MORC2 (scale bar: 200µm). B. Survival quantification of cortical neurons over time by counting eGFP soma in at least 120 fields per condition from more than 4 independent experiments. Kruskal-Wallis test followed by Dunn’s multiple comparison test. *** p<0.0001. C. Confocal images of cortical neurons at day 2 expressing MORC2 and eGFP counterstain for activated caspase 3 (in red). Scale 20µm. D. Quantification of the number of activated-caspase3 neurons per field at day 2 normalized by WT condition (70 fields from 4 independent experiments). Kruskal-Wallis test followed by Dunn’s multiple comparison test. *** p<0.0001. E. Box plot representation of the axon length distribution (at least 2500 neurons, from 120 fields, from 4 independent experiments) Kruskal-Wallis test followed by Dunn’s multiple comparison test.