Figure 3 MORC2 mutants affect SH-EP survival and apoptosis.
A. Microscope images of SH-EP culture at 3 days in vitro after MORC2 transfection. Cell are counted stained for nuclei with DAPI (in blue). Apoptotic cells are stained with anti activated-caspase 3 antibody (in red). Scale 100 µm. B. SH-EP survival quantification by counting eGFP positive cells in more than 75 fields from more than 3 independent experiments. Kruskal-Wallis test followed by Dunn’s multiple comparison test. C. Quantification of the percentage of activated-caspase 3 positive cell at day 3 and normalized by WT condition. Kruskal-Wallis test followed by Dunn’s multiple comparison test. ns p<0.05 ; * p<0.01 ; ** p<0.001 ; *** p<0.0001