2.1. Materials.
The vegetable oil (VO) used was a refined, bleached, and deodorized high
oleic safflower oil obtained from a local distributor (Coral
Internacional, San Luis Potosi, Mexico). Previous characterization
indicated that the major triacylglycerides (TAGS) in the VO used were
OOO (65.65% ± 0.15%), LOO (16.26% ± 0.04), and POO (8.58% ± 0.04),
and as minor TAGS components: StOO (2.64% ± 0.01%), LLO (2.25% ±
0.02%), POL (1.70% ± 0.11), StLL (0.87% ± 0.05%), and LLL (0.46% ±
0.01%) (O = oleic acid; L = linoleic acid; St = stearic acid; P =
palmitic acid) (Alvarez-Mitre et al., 2012). The micronized refined
candelilla wax (CW), supplied by Multiceras (Monterrey, Mexico) was
previously characterized by gas chromatography coupled with mass
spectrophotometry after derivatization of the CW with
N,O-Bis(trimethylsilyl)trifluoroacetamide following the procedure
described by Ortega-Salazar (2012). The results indicated that the main
component of the CW were n -alkanes (45.75% ± 1.01),n -aliphatic alcohols (5.27 ± 1.40),n -fatty acids (15.17% ±
2.03), long chain esters (7.45% ± 0.61),
triterpenic alcohols (23.41% ±
1.55), and esters of triterpenic alcohols (7.45% ± 0.17).