2.5. Emulsion stability through differential scanning calorimetry
The stability of the emulsions developed was evaluated through freeze-thaw cycles between 25°C and -70°C using a DSC (Discovery; TA Instruments, New Castle, USA). Initially, the emulsion samples (4 mg to 8 mg) sealed in hermetic aluminum pans were kept for 5 min at 25°C and then cooled down to -70°C (5°C/min). After 5 min at -70°C the sample was heated (5°C/min) until reached 25°C. After 5 at 25°C we repeated the freeze-thaw cycle under the same time-temperature conditions. The evaluation of the thermograms to determine the stability of the emulsions was done following the approach described by Clausse et al. (2005) and Ghosh and Rousseau (2009).