2.5. Emulsion stability through differential scanning
calorimetry
The stability of the emulsions
developed was evaluated through freeze-thaw cycles between 25°C and
-70°C using a DSC (Discovery; TA Instruments, New Castle, USA).
Initially, the emulsion samples (4 mg to 8 mg) sealed in hermetic
aluminum pans were kept for 5 min at 25°C and then cooled down to -70°C
(5°C/min). After 5 min at -70°C the sample was heated (5°C/min) until
reached 25°C. After 5 at 25°C we repeated the freeze-thaw cycle under
the same time-temperature conditions. The evaluation of the thermograms
to determine the stability of the emulsions was done following the
approach described by Clausse et al. (2005) and Ghosh and Rousseau
(2009).