2.3. Polarized light microscopy
The microstructure of the emulsions was evaluated using a microscope
(Olympus BX51, Olympus Optical Co., Ltd., Tokyo, Japan) equipped with a
video camera (KPD50, Digital Hitachi, Tokyo, Japan), a heating/cooling
stage (LTS 350, Linkam Scientific Instruments, Ltd.) connected to a
temperature control station (TP94 and Linksys32 V 1.3.1; Linkam
Scientific Instruments, Ltd., Surrey, England), and a tank of liquid
nitrogen. Maintaining the temperature of the stage at 25°C we obtained
images of the sample using polarized light.