2.10. ATP assay
The metabolic activity of bacteria was assessed by an Enhanced ATP Assay
Kit (Beyotime). K.pneumoniae cells treated with different samples
was harvested by centrifuging (8000 rpm,5 min) at 4 °C and resuspended
in 100 μL of lysis buffer. Subsequently, all samples were transferred
into an ultrasonic cell disruption instrument at 30% power (3 s on, 5 s
off) for 5 min in an ice bath. Finally, the harvested supernatant was
determined on a multi-mode detection platform in luminance mode.