Fig. 4. Observation of bacterial cell wall destruction ofK.pneumoniae. (A) Calcein-AM/PI staining of K.pneumoniae(1×108 CFU mL-1)after various
treatments. (normal saline, NIR, HMPB, HMPB + NIR, Ofloxacin, Ofloxacin
+ NIR, OHH NPs, OHH NPs + NIR). The net concentration of the HMPB
substrate in all sample groups has been maintained at 100 μg/mL. (B) The
corresponding quantitative analysis of fluorescence intensity by Image
J. (C) SEM images of K.pneumoniae cells after various treatments.