2.5. Characterization of hybrids
SEM mapping, FTIR, X-ray diffraction (XRD) and X-ray photoelectron spectroscopy (XPS) were tested in Guangdong Puchuan Inspection Co, Ltd., China. UV–Vis absorption spectra were recorded with a UV-1800 spectrophotometer (Shimadzu, Japan). The hydrodynamic diameters and zeta potential of NPs were measured using a Zeta-sizer Nano ZS (Malvern, UK). The morphology of NPs was characterized by transmission electron microscope (TEM, JEM-2100F, JEOL).
2.6. Photothermal effect ofOHH NPs
0.6 mL of PBS, HMPB NPs, ofloxacin or OHH NPs solution in Eppendorf tube was exposed to 808 nm NIR laser with a laser density of 1 W/cm2 for 10 min. An infrared thermal camera was used to capture the infrared thermal images and record the real-time temperature every 20s. To assess the photostability of OHH NPs, the real-time temperature of OHH NPs (100 μg/mL) solution was recorded in five cycles with 5 min laser on and 10 min laser off.