2.15. In vivo treatment of wound infection in Balb/c mice
All procedures for animals were approved by the Institutional Animal Care and Use Committee of Hunan University. Six-week-old female Balb/c mice were obtained from Hunan SJA Laboratory Animal Co., Ltd. (Changsha, China). K.Pneumoniae was used as bacterial strain to infect mice. All mice were randomly divided into five groups: PBS group, HMPB group, ofloxacin group, OHH group and OHH+NIR group. A circular skin wound (diameter of 10 mm) was prepared on the backbone of mice. Then, 100 μL of K.pneumoniae suspension (108 CFU mL-1) was dropped on circular wounds for mouse infection. Three days after continuous infection, 100 μL of PBS, HMPB, ofloxacin OHH was smeared on the surface of the wounds every 2 days with the same antibiotic concentration (10 μg mL−1) and the same HMPB concentration (100 μg mL−1), respectively. The OHH+NIR group was irradiated by 808 nm NIR laser (1 W/cm2) for 5 min. Meanwhile, the diameter of the wounds was measured and the wounds were photographed every 2 days.
On the 12th day, all mice were euthanized, and skin tissues and major organs were excised to study their healing efficiency and biological safety in vivo, respectively. In order to evaluate the antibacterial effect in vivo, these infected tissues were also collected for coating assay. Collect blood for measuring biochemical indicators.