4. Conclusion
In summary, the OHH NPs nanoplatform was fabricated for antibiotic-photothermal ablation of K.pneumoniae bacteria. The integrated nanoplatform could efficiently adhere ontoK.pneumoniae bacteria and damage bacterial membrane by subsequent PTT under NIR irradiation. The exploration of the antibacterial mechanism shows that the powerful bactericidal ability of the cooperative system and the inhibition of drug resistance can be attributed to the generation of ROS, which destroys the integrity of cell membranes, reduces ATP and disrupts bacterial metabolism. The outstanding bactericidal capability of the OHH NPs nanoplatform under NIR irradiation was also confirmed by an in vivoK.pneumoniae -infected model on mice. OHH NPs nanoplatform combined with antibiotic-photothermal therapy can be considered as a very promising strategy to effectively eliminate bacteria and inhibit the development of drug resistance.