3.9. In vivo biological safety study
In order to evaluate the in vivo potential toxicity of OHH NPs+NIR,
biochemical analysis of all mice with different treatments was performed
when wound healing experiment was completed. Fig. 10A-D showed that
there were no obvious differences among all groups in total white blood
cell (WBC), total red blood cells (RBC), hemoglobin (HGB) and blood
platelet (PLT) counts. Meanwhile, no abnormalities were found in key
functional hepatic and kidney indicators, such as ALT, AST, UREA and
CREA (Fig. 10E-H). In addition, H&E staining images of the major organs
including heart, liver, spleen, kidney, and lung confirmed normal tissue
structures without any visible organ damage. All these results strongly
demonstrated our developed therapeutic strategy of OHH NPs+NIR with the
superior biosafety for in vivo infected wound healing therapy.