2.11. Protein leakage study
Protein leakage detection of bacteria was performed referring to our previous method. In brief, 108 CFU mL-1 K.pneumoniae was firstly incubated with PBS (control), HMPB (100 μg/mL), ofloxacin (10 μg/mL), and OHH NPs (100 μg/mL) for 12 h at 37 °C. Hereafter, bacterial suspension ofK.pneumoniae was centrifuged at 8000 rpm for 5 min at 4 °C and the harvested supernatant was immediately transferred into a 96-well plate. The concentration of protein was determined using a BCA Protein Assay Kit (Cat#PC0020, Solarbio).