3.9. In vivo biological safety study
In order to evaluate the in vivo potential toxicity of OHH NPs+NIR, biochemical analysis of all mice with different treatments was performed when wound healing experiment was completed. Fig. 10A-D showed that there were no obvious differences among all groups in total white blood cell (WBC), total red blood cells (RBC), hemoglobin (HGB) and blood platelet (PLT) counts. Meanwhile, no abnormalities were found in key functional hepatic and kidney indicators, such as ALT, AST, UREA and CREA (Fig. 10E-H). In addition, H&E staining images of the major organs including heart, liver, spleen, kidney, and lung confirmed normal tissue structures without any visible organ damage. All these results strongly demonstrated our developed therapeutic strategy of OHH NPs+NIR with the superior biosafety for in vivo infected wound healing therapy.