Abstract
Bacterial infections, especially infections caused by multi-drug
resistant bacteria, pose a serious threat to human health and bring huge
challenges to clinical treatment. The excessive use of antibiotics can
easily lead to the emergence of bacterial resistance, which severely
limits clinical treatment options. There is an urgent need to develop
high-efficiency antibacterial materials and treatment strategies to
inhibit infections caused by multidrug-resistant bacteria. In this work,
a nanocomposite named Ofloxacin@HMPB@HA(OHH NPs) combined with the laser
irradiation was used to reduce the development of drug resistance and
accelerate wound healing in a model infected by Klebsiella
pneumoniae(K.Pneumoniae) . In vitro results showed that compared with
OHH NPs or NIR laser irradiation alone, this combination strategy can
exert a synergistic effect on anti-K.Pneumoniae by destroying
cell integrity with generating ROS and reducing ATP, and also inhibit
the development of bacterial resistance. Moreover, in vivo experiments
have shown that the system effectively promotes wound healing through
killing K.Pneumoniae and promoting the formation of new tissues. In
summary, these results indicate that OHH NPs show great potential in the
clinical application of bacterial infections.