Characterization of primary cultured BM ECs
On the 7th day of culture, anchorage-dependent spindle-shaped cells were trypsinized and then confirmed by flow cytometry using the CD34+CD309+CD133+markers. In addition, the spindle-shaped cells with double-positive staining of DiI-Ac-LDL and FITC-UEA-I were functionally determined to be ECs (Fig. 2C).