3.5. Increased DPSCs viability on scaffolds coated with PLL
As DPSCs viability was not affected by seeding density conditions,
densities 1, 3 and 5 were selected to conduct AB analysis over 24- and
48-hours. In order to determine the effects of coating conditions on
seeded DPSCs, three different coating conditions (NC, PLL, and PLL+LAM)
were used. The effect of cell seeding densities was impacted by various
coating conditions following 24 hours of culture. Significantly higher
AB reduction percentage was observed for SA and GOSA scaffolds at all
three densities compared to the 2D control condition (Fig. 4a-c). After
48 h of DPSCs culture, statistically significant differences were
detected in the proliferation profiles of cells growing in 2D and 3D
under various coating conditions (Fig. 4d-f). In addition, PLL coating
significantly increased the cellular activity of GOSA scaffolds at all
three cell densities within 48 hours of DPSCs culture (Fig. 4).