3.5. Increased DPSCs viability on scaffolds coated with PLL
As DPSCs viability was not affected by seeding density conditions, densities 1, 3 and 5 were selected to conduct AB analysis over 24- and 48-hours. In order to determine the effects of coating conditions on seeded DPSCs, three different coating conditions (NC, PLL, and PLL+LAM) were used. The effect of cell seeding densities was impacted by various coating conditions following 24 hours of culture. Significantly higher AB reduction percentage was observed for SA and GOSA scaffolds at all three densities compared to the 2D control condition (Fig. 4a-c). After 48 h of DPSCs culture, statistically significant differences were detected in the proliferation profiles of cells growing in 2D and 3D under various coating conditions (Fig. 4d-f). In addition, PLL coating significantly increased the cellular activity of GOSA scaffolds at all three cell densities within 48 hours of DPSCs culture (Fig. 4).