2.1. Materials
Sodium alginate and Calcium chloride dried were purchased from
Chem-Supply. Alpha-MEM (Gibco, Life Technologies, Australia, Cat. No.
12561056) was supplemented with fetal bovine serum (FBS, USA origin,
Life Technologies, Australia), penicillin /streptomycin (Gibco),
L-ascorbic acid-2-phosphate (Sigma-Aldrich), and 2mM L-Glutamine (Life
Technologies, Australia). Natural mouse Laminin (Gibco-Life
Technologies) and 0.01% Poly-l-lysine (PLL) solution (Sigma) were used
as coating reagents. Trypsin-EDTA was supplied by Gibco Life
Technologies, Australia. Clear-bottom 24-well and 96-well
plates (Costar®, Corning, NY, USA) were used throughout the study. The
cytotoxicity assay was performed following the manufacturer’s protocol
using LDH kit from Promega Corporation (WI, USA). The
AlamarBlueTM cell viability reagent was supplied by
Invitrogen–ThermoFisher Scientific, USA.