Statistical analysis
Data are expressed as mean ± SEM. Statistical comparisons between
experimental groups were performed using one-way ANOVA and post-hoc
Tukey or Dunnett’s multiple comparison tests. For less than three
experimental groups, Student’s unpaired two-tailed t -test was
employed. Data were analyzed with Microsoft Excel (Microsoft, Redmond,
Washington, USA) and Prism 6.0 (GraphPad Software, San Diego,
California). A ‘p ’ value of less than 0.05 was considered
statistically significant for all analyses.