OVA/SEB allergic peritonitis induces production of OVA and SEB specific IgE.
Next, we evaluated levels of OVA specific IgE in the serum of AP induced WT mice 48h after challenge. We found these antibodies to be increased, although not significantly, in WT OVA/SEB challenged mice in comparison to PBS challenged (Fig. 3A). In order to check OVA specific IgE together with SEB specific IgE, we analyzed WT BMMCs sensitized with serum retrieved from OVA/SEB challenged mice and activated them with either OVA or SEB. Our results show that, under these conditions, BMMCs were specifically activated by either OVA (significantly) or SEB (not significantly) to release TNFα. (Fig. 3B). These data reconfirm that OVA/SEB mice produce IgE specific for both the allergens.