OVA/SEB allergic peritonitis induces production of OVA and SEB
specific IgE.
Next, we evaluated levels of OVA specific IgE in the serum of AP induced
WT mice 48h after challenge. We found these antibodies to be increased,
although not significantly, in WT OVA/SEB challenged mice in comparison
to PBS challenged (Fig. 3A). In order to check OVA specific IgE together
with SEB specific IgE, we analyzed WT BMMCs sensitized with serum
retrieved from OVA/SEB challenged mice and activated them with either
OVA or SEB. Our results show that, under these conditions, BMMCs were
specifically activated by either OVA (significantly) or SEB (not
significantly) to release TNFα. (Fig. 3B). These data reconfirm that
OVA/SEB mice produce IgE specific for both the allergens.