Allergic Peritonitis (AP)
8-9-week-old C57BL/6 (WT) females and/or males were sensitized on days -14 and -7 with subcutaneous (s.c) injection of 100μg of OVA (A5378-10G, Sigma-Aldrich, St. Louis, MO, USA) + 1μg of SEB (S4881-5mg, Sigma-Aldrich) in 200μl of PBS (#1927718, BI, Beit-Haemek, IL) per mouse (OVA [500μg/ml]/SEB [5 μg/ml]) and challenged at day 0 with i.p injection of 10μg of OVA + 0.1μg SEB in 200μl of PBS per mouse (OVA [50µg/ml]/SEB [0.5 µg/ml]) or PBS only. Mice were euthanized by CO2 inhalation on days 0 (0h), 2 (48h), 4 (96h), 7(168h) and 10 (240h) and peritoneal lavage was performed with 3 ml PBSX1+3% FCS. Peritoneal cells were counted in hemocytometer by Trypan blue (#03-102-1B, BI) staining. Peritoneal lavages were centrifuged at 150g, 5min, 4oC and supernatants were collected and stored or immediately used for further analysis. Cell pellets were resuspended in Flow Cytometry (FC) buffer (0.5% BSA + 2% FCS in PBSX1) for FC analysis.