Vascular Permeability
The OVA/SEB AP model was induced and 1h before the challenge, mice were
injected intravenously (i.v.) with FITC-Dextran (#46945-100mg,
Sigma-Aldrich), 0.3mg / 150µl in PBS per mice. Challenge was then
performed, and mice were euthanized 30 min later and peritoneal lavage
(3ml) was performed. Cells were separated from supernatants by
centrifugation and supernatants were analyzed for FITC-Dextran levels in
Cytation 3 Cell Imaging Multi-Mode Reader (BioTek Instruments, Inc;
Winooski, VT, USA) using the following parameters: Excitation: 490 nm,
Emission: 520 nm.