Tryptase and cytokines levels in peritoneal lavage
For assessing tryptase and cytokine levels in the peritoneal cavity, OVA/SEB AP-induced and challenged mice were euthanized 30 min or 48h after challenge respectively and peritoneal lavages (1 ml) were collected. Cells were separated from supernatants by centrifugation and supernatants were cleared by any remaining cell by a second centrifugation step. Tryptase levels were assayed as described before (15). Cytokines levels were determined by using IL-4 (900-K49; PeproTech, Rehovot, IL), INFɣ (900-K98; PeproTech) and IL-17A (900-K392; PeproTech) ELISA kits according to the manufacturer’s instructions.