4. Conclusion

In this study, we developed a semi-automatic approach for accurate and efficient construction of metabolic models of multiple SRP species by applying pan-genome analysis and the concept of core metabolic model. The constructed core metabolic model for DvH can successfully predict growth yield and several growth characteristics. Flux balance analysis of the SRP core metabolic models under different respiratory conditions has given birth to an overall energy conservation model. In addition to the previously reported hydrogen cycling model, the functions of various membrane-bound electron transfer complexes in energy conservation were elucidated, the recently proposed FBEB mechanism for energy metabolism was also supported in this study. Such information is essential for understanding the versatile metabolic capacities of SRPs and for developing SRP-based biotechnologies. More importantly, the present work developed an efficient approach for construction of multiple metabolic models, which creates an opportunity to explore and manipulate the metabolism of key microbes in the environment and biotechnology.