4. Conclusion
In this study, we developed a semi-automatic approach for accurate and
efficient construction of metabolic models of multiple SRP species by
applying pan-genome analysis and the concept of core metabolic model.
The constructed core metabolic model for DvH can successfully predict
growth yield and several growth characteristics. Flux balance analysis
of the SRP core metabolic models under different respiratory conditions
has given birth to an overall energy conservation model. In addition to
the previously reported hydrogen cycling model, the functions of various
membrane-bound electron transfer complexes in energy conservation were
elucidated, the recently proposed FBEB mechanism for energy metabolism
was also supported in this study. Such information is essential for
understanding the versatile metabolic capacities of SRPs and for
developing SRP-based biotechnologies. More importantly, the present work
developed an efficient approach for construction of multiple metabolic
models, which creates an opportunity to explore and manipulate the
metabolism of key microbes in the environment and biotechnology.