2.1 Strains, media and culture
All strains used in this study are listed in Table S1. Z. mobilisstocks were inoculated into the rich medium (RM) composed of 10 g/L
yeast extract, 20 g/L glucose, and 2 g/L
KH2PO4, grown statically at 30 °C until
their exponential phase, and then transferred into 250 mL flasks, each
containing 100 mL RM medium with 10% inoculation for subculture to
increase OD600 to ~1.0. When needed, 20
µg/mL tetracycline was added to the RM medium. Congo red staining was
used to visualize the cellulose produced by Z. mobili s, and 2 µL
of the subculture was inoculated onto RM agar plates containing 70 µ
µg/mL Congo red, which were incubated for 12−24 h at 30 °C for visual
inspection (Trivedi et al., 2016; Thongsomboon et al., 2020).