Transcriptomic analysis
High-resolution genome-wide transcriptional analysis with RNA-Seq was
carried out by Beijing Genomics Institute (BGI-Huada, Shenzhen, China).
Raw reads were filtered to remove some adapters and low-quality reads,
and the remaining reads were mapped to the reference genome
S288c_reference_genome_R6-1-1_20110203 to get the transcripts
information. Next, the expression levels of the differentially expressed
genes (DEGs) were normalized by RPKM, defined as the number of reads per
kilobase per million mapped reads. To determine the DEGs in different
periods, absolute value of log2-fold changes larger than
1 and false discovery rate lower than 0.01 were selected as screening
criteria.