Confirmation of transcriptomic results by qRT-PCR
To determine the copy numbers of the transcripts, quantitative real-time
PCR (qRT-PCR) was conducted for each sample. Six representative genes,ZPS1 (zinc regulatory proteins), DSE1 (cell division),HPF1 (mannose glycoprotein), EGT2 (cell division),TIS11 (iron regulatory proteins) and, GCV1 (synthesis of
coenzyme factors) were selected to perform the verification. The primers
are shown in Table S1.