2.1 Materials and enzymes used
Unless noted, the materials used were reported previously (Goedl et al., 2007). Lm SucP (GenBank identifier BAA14344) and Ba SucP (GenBank identifier AF543301.1) were isolated from Escherichia coli overexpression cultures according to literature (Goedl et al., 2007). Their specific activities (phosphorolysis of sucrose) were 166 U/mg (Lm SucP) and 108 U/mg (Ba SucP), consistent with the previous studies (Goedl et al., 2007; van den Broek et al., 2004). Protein was measured with Bradford assay referenced against BSA.