On the donor substrate dependence of group transfer reactions by
hydrolytic enzymes: insight from kinetic analysis of sucrose
phosphorylase-catalyzed transglycosylation
Mario Klimacek1¶, Alexander Sigg1¶,
and Bernd Nidetzky1,2,*
1Institute of Biotechnology and Biochemical
Engineering, Graz University of Technology, NAWI Graz,
Petersgasse 10-12/I, A-8010 Graz,
Austria
2Austrian Centre
of Industrial Biotechnology (acib),
Krenngasse 37, A-8010 Graz,
Austria
* Corresponding author (B.N.):
bernd.nidetzky@tugraz.at;
+43-316-873-8400
¶ These authors contributed equally.
Abbreviations used : G1P,
α-D-glucose 1-phosphate; GG,
α-glucosyl glycerol; E-Glc, β-glucosyl-enzyme intermediate; SucP,
sucrose phosphorylase (EC 2.4.1.7); Ba SucP, SucP fromBifidobacterium adolescentis ; Lm SucP, SucP from