2.1 Materials and enzymes used
Unless noted, the materials used
were reported previously (Goedl et al., 2007). Lm SucP (GenBank
identifier BAA14344) and Ba SucP (GenBank identifier AF543301.1)
were isolated from Escherichia coli overexpression cultures
according to literature (Goedl et al., 2007). Their specific activities
(phosphorolysis of sucrose) were 166 U/mg (Lm SucP) and 108 U/mg
(Ba SucP), consistent with the previous studies (Goedl et al.,
2007; van den Broek et al., 2004). Protein was measured with Bradford
assay referenced against BSA.