On the donor substrate dependence of group transfer reactions by hydrolytic enzymes: insight from kinetic analysis of sucrose phosphorylase-catalyzed transglycosylation
Mario Klimacek, Alexander Sigg, and Bernd Nidetzky1,2,*
1Institute of Biotechnology and Biochemical Engineering, Graz University of Technology, NAWI Graz, Petersgasse 10-12/I, A-8010 Graz, Austria
2Austrian Centre of Industrial Biotechnology (acib), Krenngasse 37, A-8010 Graz, Austria
* Corresponding author (B.N.): bernd.nidetzky@tugraz.at; +43-316-873-8400
These authors contributed equally.
Abbreviations used : G1P, α-D-glucose 1-phosphate; GG, α-glucosyl glycerol; E-Glc, β-glucosyl-enzyme intermediate; SucP, sucrose phosphorylase (EC 2.4.1.7); Ba SucP, SucP fromBifidobacterium adolescentis ; Lm SucP, SucP from