Table 1. Apparent kinetic parameters for LmSucP and BaSucP obtained from fits of experimental data and calculated using rate constants for mechanisms M1 and M3. Table 1. Apparent kinetic parameters for LmSucP and BaSucP obtained from fits of experimental data and calculated using rate constants for mechanisms M1 and M3. Table 1. Apparent kinetic parameters for LmSucP and BaSucP obtained from fits of experimental data and calculated using rate constants for mechanisms M1 and M3. Table 1. Apparent kinetic parameters for LmSucP and BaSucP obtained from fits of experimental data and calculated using rate constants for mechanisms M1 and M3. Table 1. Apparent kinetic parameters for LmSucP and BaSucP obtained from fits of experimental data and calculated using rate constants for mechanisms M1 and M3. Table 1. Apparent kinetic parameters for LmSucP and BaSucP obtained from fits of experimental data and calculated using rate constants for mechanisms M1 and M3. Table 1. Apparent kinetic parameters for LmSucP and BaSucP obtained from fits of experimental data and calculated using rate constants for mechanisms M1 and M3.
LmSucP LmSucP LmSucP BaSucP BaSucP BaSucP
Data fit Kinetic mechanism e Kinetic mechanism e Data fit Kinetic mechanism
Sucrose, M1 Sucrose, M1
[GOH] = 2 M [GOH] = 2 M [GOH] = 2 M [GOH] = 2 M [GOH] = 2 M
\({}^{\text{app}}V_{X}\) [s-1] \({}^{\text{app}}V_{X}\) [s-1] 23.3 ± 0.45 a 23.2 23.2 15.9 ± 0.1 a 15.3
\({}^{\text{app}}K_{\text{Suc}}\) [mM] \({}^{\text{app}}K_{\text{Suc}}\) [mM] 1.4 ± 0.1 a 1.0 1.0 0.45 ± 0.01 a 0.46
[Suc] = 20 mM [Suc] = 20 mM [Suc] = 20 mM [Suc] = 20 mM [Suc] = 20 mM
\(v_{H}^{\left[\text{GOH}\right]=0}\) [s-1] \(v_{H}^{\left[\text{GOH}\right]=0}\) [s-1] 2.1 ± 0.3 b 2.68 2.68 0.5 ± 0.1 b 0.88
\(\frac{{}^{\text{app}}V_{X}}{{}^{\text{app}}K_{\text{GOH}}}\) [M-1s-1] \(\frac{{}^{\text{app}}V_{X}}{{}^{\text{app}}K_{\text{GOH}}}\) [M-1s-1] 10.7 ± 0.2 b 12.3 12.3 7.1 ± 0.1 b 7.2
TC [M-1] TC [M-1] 4.7 ± 0.1 c 4.7 4.7 7.9 ± 0.1 c 8.2
G1P, M3 G1P, M3
[GOH] = 2 M [GOH] = 2 M [GOH] = 2 M [GOH] = 2 M [GOH] = 2 M
\({}^{\text{app}}V_{X}\) [s-1] \({}^{\text{app}}V_{X}\) [s-1] 36 ± 3 d 28.5 28.5 29.2 ± 0.7 a 25.8
\({}^{\text{app}}K_{G1P}\) [mM] \({}^{\text{app}}K_{G1P}\) [mM] 12.5 ± 1.7 d 7.6 7.6 58 ± 5 a 48.5
\({}^{\text{app}}K_{i,G1P}\) [mM] \({}^{\text{app}}K_{i,G1P}\) [mM] 106 ± 21 d n.d.f n.d.f none none
[G1P] = 50 mM [G1P] = 50 mM [G1P] = 50 mM [G1P] = 450 mM [G1P] = 450 mM
\({}^{\text{app}}V_{X}\) [s-1] \({}^{\text{app}}V_{X}\) [s-1] 122 ± 48 b 73.9 73.9 144 ± 66 b 238
\(v_{H}\) [s-1] \(v_{H}\) [s-1] 3.0 ± 0.3 b 3.20 3.20 1.9 ± 0.4 b 2.0
\({}^{\text{app}}K_{\text{GOH}}\) [M] \({}^{\text{app}}K_{\text{GOH}}\) [M] 11.0 ± 0.5 b 6.6 6.6 9.8 ± 5.4 b 19.6
\(\frac{{}^{\text{app}}V_{X}}{{}^{\text{app}}K_{\text{GOH}}}\) [M-1s-1] \(\frac{{}^{\text{app}}V_{X}}{{}^{\text{app}}K_{\text{GOH}}}\) [M-1s-1] 11.1 b 11.2 11.2 14.6 b 12.1
TC [M-1] TC [M-1] 3.3 ± 0.1 c 3.65 3.65 6.3 ± 0.1 c 6.0
a Eq.1, b Eq. 3, c Eq. 4, d Eq. 2 e Results were calculated with \(k_{+4}\) = 195.15 s-1. f A term representing \({}^{\text{app}}K_{i,G1P}\) in Eq. 2 cannot be isolated from the rate equation of M3. a Eq.1, b Eq. 3, c Eq. 4, d Eq. 2 e Results were calculated with \(k_{+4}\) = 195.15 s-1. f A term representing \({}^{\text{app}}K_{i,G1P}\) in Eq. 2 cannot be isolated from the rate equation of M3. a Eq.1, b Eq. 3, c Eq. 4, d Eq. 2 e Results were calculated with \(k_{+4}\) = 195.15 s-1. f A term representing \({}^{\text{app}}K_{i,G1P}\) in Eq. 2 cannot be isolated from the rate equation of M3. a Eq.1, b Eq. 3, c Eq. 4, d Eq. 2 e Results were calculated with \(k_{+4}\) = 195.15 s-1. f A term representing \({}^{\text{app}}K_{i,G1P}\) in Eq. 2 cannot be isolated from the rate equation of M3. a Eq.1, b Eq. 3, c Eq. 4, d Eq. 2 e Results were calculated with \(k_{+4}\) = 195.15 s-1. f A term representing \({}^{\text{app}}K_{i,G1P}\) in Eq. 2 cannot be isolated from the rate equation of M3. a Eq.1, b Eq. 3, c Eq. 4, d Eq. 2 e Results were calculated with \(k_{+4}\) = 195.15 s-1. f A term representing \({}^{\text{app}}K_{i,G1P}\) in Eq. 2 cannot be isolated from the rate equation of M3. a Eq.1, b Eq. 3, c Eq. 4, d Eq. 2 e Results were calculated with \(k_{+4}\) = 195.15 s-1. f A term representing \({}^{\text{app}}K_{i,G1P}\) in Eq. 2 cannot be isolated from the rate equation of M3.