2.6 Sugar measurements
The sample supernatant was pipetted into a 96-well plate at 250 µL and placed in the YSI 2900 Biochemistry Analyzer (Xylem, Inc., Rye Brook, NY) to measure sucrose and glucose concentrations in the culture medium. To ensure the sugar concentrations were within the calibration ranges, samples were sometimes diluted with distilled water prior the measurement.