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Figure 1. Upstream process flowsheet for the two-stage semicontinuous operation base case scenario in the SuperPro Designer® model.
Figure 2. Two-stage semicontinuous operation of transgenic rice cell culture with the RAmy3D expression system.
Figure 3. Downstream process flowsheet for the two-stage semicontinuous operation base case scenario in the SuperPro Designer® model.
Figure 4. Results of the base case scenario analysis. (A) Tornado chart displaying sensitivity of rrBChE cost of goods sold (COGS) to variation in the tested process parameters over the selected analysis range. Individual scenario analyses of (B) expression level, (C) harvest size, and (D) Hupresin capacity variation on rrBChE COGS. (E) Variation in COGS breakdown in the three scenario analyses. The simulated facility is re-sized for each scenario analysis result to maintain base case production level and concentration in product formulation. Values corresponding to the base case are circled in black. FW, fresh weight.
Figure 5. Annual operating expenditures for production of rrBChE in two-stage semicontinuous and two-stage batch mode operation using a contract manufacturing organization and with a greenfield single-product facility broken down by (A) cost items, and (B) manufacturing section. Depreciation costs are not included in the annual operating costs for the new facility scenarios.
Figure 6. Total equipment costs for a greenfield single-product facility producing rrBChE in two-stage semicontinuous and two-stage batch mode operation broken down by manufacturing section.
Figure 7. (A) Sensitivity analysis of rrBChE cost of goods sold to the cost of culture media for batch mode operation using two-stage (medium exchange) and single-stage (simple) induction strategies for new greenfield single-product facility design including depreciation costs. (B) The variation in operating cost contributions as a function of culture medium cost. The base case scenario price is at $0.10/L. PBR, production bioreactor.