Figure 4 Multiplex genome editing using the pCBEso system in S. oneidensis MR-1. (a) Map of the pCBEso-blaA -dmsE plasmid simultaneously targetingblaA and dmsE . (b) Sanger sequencing of two targeted regions within blaA and dmsE genes. The protospacer is shown in red wireframe, and amino acids with their codons are indicated. PAM sequence is highlighted in red bold font. The number in red indicates positively edited clones, while the number in black represents total sequenced clones randomly picked from the transformation plates.
Figure 5 (a) GlcNAc and the predicted glucose utilization pathway in S. oneidensis MR-1. Growth curves of the wild-type and edited strains cultured in mineral medium using GlcNAc (b) or glucose (d) as the sole carbon source. (c) The transcription levels of genes associated with the uptake and metabolism of GlcNAc or glucose innagR BE relative to those in S. oneidensisMR-1.
Figure 6 Anaerobic reduction of methyl orange (a), amaranth (b), and roxarsone (c) using glucose as the sole carbon source by the wild-type and engineered strain nagR BE. 4-amino-benzenesulfonic acid (4-ABA), 4-nitro-benzene sulfonate (4-NBS) was one product of methyl orange and amaranth reduction, respectively. HAPA(V), the main product of roxarsone reduction. The first-order rate constants (k ) were used to evaluate the reduction rates of different organic pollutants.
Figure 7 Anaerobic reduction of methyl orange (a), amaranth (b), and roxarsone (c) using GlcNAc as the sole carbon source by the wild-type and engineered strain nagR BE. 4-amino-benzenesulfonic acid (4-ABA), 4-nitro-benzene sulfonate (4-NBS) was one product of methyl orange and amaranth reduction, respectively. HAPA(V), the main product of roxarsone reduction. The first-order rate constants (k ) were used to evaluate the reduction rates of different organic pollutants.