System Host (accumulation region) ACEI peptide (s) Construct strategy Administration mode Anti-HT (mmHg) Dose (g.kg-1) Ref.
Whole transgenic plant
Rice (seeds)
Novokinin (RPLKPW)
Fusion with glutelin B1 (pGluB1-HRP) 2x Novokinin in 2 HVR of GluB1 acidic subunit plus 1x Novokinin in the C terminal VR of GluB1 basic subunit
oral administration of crude glutelin B1 fraction
-28±7 (4h)
0.03
(Yang et al., 2006)
oral administration of unpolished pulverized GluB1-HRP rice seeds Not significant 1.00
Fusion with glutelin A2 and C (pGluA2-HRP/GluC-HRP) 2x Novokinin in 2 HVR of GluA2 and GluC acidic subunits
oral administration of unpolished pulverized GluA2-HRP/GluC-HRP rice seeds
-15.6±4.8 (2h)
1.00
Tandem structure 10x Novokinin + KDEL; 18x Novokinin + KDEL
oral administration of transgenic seeds containing 18x novokinin
-15.6±1.1 (4h)
1.00
(Wakasa et al., 2011)
Soybean (seeds)
Novokinin (RPLKPW)
Fusion with β-conglycinin α’ subunit 1x Novokinin in 4 sites
oral administration of β-conglycinin fraction
-16.0±1.5 (8h)
0.15
(Yamada et al., 2008)
oral administration of defatted flour -16.4±4.3 (6h) 0.25
Fusion with β-conglycinin α’ subunit 4x Novokinin
ND
ND
ND
(Nishizawa et al., 2008)
Tomato (fruits)
VY
Fusion with acidic subunit of amarantin 4x VY + KDEL
ND
ND
ND
(Germán-Báez et al., 2014)
Cell suspension cultures
Tobacco (calli)
VY
Fusion with acidic subunit of amarantin 4x VY + KDEL
ND
ND
ND
(Santos-Ballardo et al., 2013)
Transplastomic
C. reinhardtii (chloroplasts)
VLPVP
Tandem structure 6x VLPVP
Oral administration of freeze-dried biomass
-20 (6h)
0.30
(Ochoa-Mendez et al., 2016)
GLDIQK VAGTWY LDAQSAPLRLLQK VLVLDTDYK CMENSA ALPMHIR
Multivariate chimeric peptide
ND
ND
ND
(Campos-Quevedo et al., 2013)