YG-1 (GHKIATFQER)
|
Tandem repeats
9, 18 or 27x YG-1 linked by the cleavage site of clostripain;
|
105 (5h)
|
= natural YG-1
|
ND
|
ND
|
(Park et al., 1998)
|
KVLPVP
|
Tandem repeats
6x KVLPVP linked by the cleavage site of clostripain; GST tag
|
170 (5h)
|
4.6
|
-21.4 (4h)
|
0.3
|
(Liu et al., 2007)
|
Tuna AI (PTHIKWGD)
|
Tandem repeats
6x Tuna AI
|
105–115 (8h)
|
2.0
|
ND
|
ND
|
(Fida et al., 2009)
|
|
Tandem repeats
4, 8, 16 or 32x Tuna AI; N and C- terminal His tag
|
218.9 (5h)
|
1.2
|
-36.5 (4h)
|
2.0
|
(Li et al., 2015)
|
IYPR
|
Tandem repeats
7xIYPR linked by the cleavage site of trypsin; N-terminal enterokinase
cleavage site; N-terminal His tag
|
1.8 (7h)1
|
111.3
|
-50 (4h)
|
0.4
|
(Huang et al., 2012)
|
GVYPHK
|
Tandem repeats
10x GVYPHK linked by the cleavage site of trypsin; N-terminal His
tag
|
13.8 (5h)
|
ND
|
-42.6 (2h)
|
0.8
|
(Wang et al., 2015)
|
HHL
|
Tandem + Fusion with Ubiquitin
40xHHL; C- terminal His tag
|
6.2 (4h)
|
8.4
|
ND
|
ND
|
(Jeong et al., 2007)
|
CEI12 (FFVAPFPEVFGK)
|
Fusion with DHFR
1xCEI12
|
0.5 (5h)2
|
ND
|
ND
|
ND
|
(Lv et al., 2003)
|
|
Fusion with pediocin leader peptide
1xCEI12
|
ND
|
ND
|
ND
|
ND
|
(Renye and Somkuti, 2015)
|
IW
|
Tandem + Fusion with MBP
16xIW; N-terminal MBP affinity tag
|
0.51 (7h)1
|
1.723
5.154
11.085
|
ND
|
ND
|
(Michelke et al., 2018)
|
IY and VKY
|
Tandem + Fusion with GST
2, 4 or 6x IYVKY linked by the cleavage site of chymotrypsin; N-terminal
His tag
|
38 (4h)6
|
18.536
|
ND
|
ND
|
(Oh et al., 2002)
|
BP1 (SLVYPFPGPI)
BP2 (NIPPLTQTPV)
BP3 (DKIHPF)7
|
Single peptides + Fusion with GST
1xBP1, 1xBP2 or 1xBP3 flanked by the cleavage sites of a proteinase from
L. helveticus PR4
|
1.5 (6h)
|
BP1 467.03
BP2 807.18
BP3 1729.21
|
ND
|
ND
|
(Losacco et al., 2007)
|
BP1, BP2 and BP3
Pro-BP1, Pro-BP2, Pro-BP3
|
Single peptides + Fusion with RBS
1xBP1, 1xBP2 or 1xBP3 and their precursor forms, fused to Shine-Dalgarno
RBS sequence for B. pseudocatenulatum at the 5’ end
|
NR
|
ND (increased in extracts)
|
ND
|
ND
|
(Losurdo et al., 2013)
|
VWIS, VW, RIY, IY, LW, IKW, LKPNM, LKP, RPLKPW, NMAINPSK, IPP
|
Multimer of ACEIPs
tandem multimer of 11 ACEIP linked by cleavage sites of gastrointestinal
proteases; GST tag
|
399 (5h)2
|
332
|
ND
|
ND
|
(Rao et al., 2009)
|
MRW, WIR, IRA, AMK, MKR, RGY, VAW, DGL, IPP, IKP, IKPFR, IW, IKPVA, AKF,
IQY VAF, VSV, IVY
|
Multimer of bioactive peptides
tandem multimer of 18 ACEIP and 9 antioxidant peptides linked by
cleavage sites of gastrointestinal proteases; N-terminal cELP-SUMO
tag
|
167.2 (20h)2
|
0.282
|
ND
|
ND
|
(Rao et al., 2016)
|
Novokinin (RPLKPW)
|
Modified β-conglycinin α’ subunit
1x Novokinin in 3 homologous sites of the core domain flanked by trypsin
and/or chymotrypsin cleavage sites
|
5.2 (42h)2
|
ND
|
-25 (4h)2
|
102
|
(Matoba et al., 2001a)
|
|
Modified β-conglycinin α’ subunit
1x Novokinin in 3 homologous sites of the core domain plus 1x Novokinin
in a homologous site of the extension domain; flanked by
gastrointestinal proteases cleavage sites
|
ND
|
ND
|
-18 (4h)2
|
2.52
|
(Onishi et al., 2004)
|
VY
|
Modified amarantin acidic subunit
4xVY in the third variable region (VR3); C-terminal His tag
AACM.3
|
60 (3h)2
|
2.02
|
ND
|
ND
|
(Luna-Suárez et al., 2010)
|
|
Modified amarantin acidic subunit
4xVY in the fourth variable region (VR4); C-terminal His tag
AACM.4
|
560 (6h)2
|
ND
|
ND
|
ND
|
(Morales-Camacho et al., 2016)
|
VY and RIPP
|
Modified amarantin acidic subunit
4xVY in VR3 and 1xRIPP in VR4; C-terminal His tag AACM3.4
|
50 (3h)2
|
1.412
|
ND
|
ND
|
(Castro-Martínez et al., 2012)
|
VY and IPP
|
Modified amarantin acidic subunit
4xVY in VR3 and 1xIPP in VR4; C-terminal His tag AMC3
|
ND
|
56402
|
-58 (4.5h)2
|
1002
|
(Medina-Godoy et al., 2013)
|