Peptide(s)
Construct strategy
[peptide] (mg.L-1)b
ACEI IC50 (μM)
Anti-HT (mmHg)
Dose (mg.kg-1)c
Ref.
YG-1 (GHKIATFQER)
Tandem repeats 9, 18 or 27x YG-1 linked by the cleavage site of clostripain;
105 (5h)
= natural YG-1
ND
ND
(Park et al., 1998)
KVLPVP
Tandem repeats 6x KVLPVP linked by the cleavage site of clostripain; GST tag
170 (5h)
4.6
-21.4 (4h)
0.3
(Liu et al., 2007)
Tuna AI (PTHIKWGD)
Tandem repeats 6x Tuna AI
105–115 (8h)
2.0
ND
ND
(Fida et al., 2009)
Tandem repeats 4, 8, 16 or 32x Tuna AI; N and C- terminal His tag
218.9 (5h)
1.2
-36.5 (4h)
2.0
(Li et al., 2015)
IYPR
Tandem repeats 7xIYPR linked by the cleavage site of trypsin; N-terminal enterokinase cleavage site; N-terminal His tag
1.8 (7h)1
111.3
-50 (4h)
0.4
(Huang et al., 2012)
GVYPHK
Tandem repeats 10x GVYPHK linked by the cleavage site of trypsin; N-terminal His tag
13.8 (5h)
ND
-42.6 (2h)
0.8
(Wang et al., 2015)
HHL
Tandem + Fusion with Ubiquitin 40xHHL; C- terminal His tag
6.2 (4h)
8.4
ND
ND
(Jeong et al., 2007)
CEI12 (FFVAPFPEVFGK)
Fusion with DHFR 1xCEI12
0.5 (5h)2
ND
ND
ND
(Lv et al., 2003)
Fusion with pediocin leader peptide 1xCEI12
ND
ND
ND
ND
(Renye and Somkuti, 2015)
IW
Tandem + Fusion with MBP 16xIW; N-terminal MBP affinity tag
0.51 (7h)1
1.723 5.154 11.085
ND
ND
(Michelke et al., 2018)
IY and VKY
Tandem + Fusion with GST 2, 4 or 6x IYVKY linked by the cleavage site of chymotrypsin; N-terminal His tag
38 (4h)6
18.536
ND
ND
(Oh et al., 2002)
BP1 (SLVYPFPGPI) BP2 (NIPPLTQTPV) BP3 (DKIHPF)7 Single peptides + Fusion with GST 1xBP1, 1xBP2 or 1xBP3 flanked by the cleavage sites of a proteinase from L. helveticus PR4
1.5 (6h)
BP1 467.03 BP2 807.18 BP3 1729.21
ND
ND
(Losacco et al., 2007)
BP1, BP2 and BP3 Pro-BP1, Pro-BP2, Pro-BP3 Single peptides + Fusion with RBS 1xBP1, 1xBP2 or 1xBP3 and their precursor forms, fused to Shine-Dalgarno RBS sequence for B. pseudocatenulatum at the 5’ end
NR
ND (increased in extracts)
ND
ND
(Losurdo et al., 2013)
VWIS, VW, RIY, IY, LW, IKW, LKPNM, LKP, RPLKPW, NMAINPSK, IPP
Multimer of ACEIPs tandem multimer of 11 ACEIP linked by cleavage sites of gastrointestinal proteases; GST tag
399 (5h)2
332
ND
ND
(Rao et al., 2009)
MRW, WIR, IRA, AMK, MKR, RGY, VAW, DGL, IPP, IKP, IKPFR, IW, IKPVA, AKF, IQY VAF, VSV, IVY
Multimer of bioactive peptides tandem multimer of 18 ACEIP and 9 antioxidant peptides linked by cleavage sites of gastrointestinal proteases; N-terminal cELP-SUMO tag
167.2 (20h)2
0.282
ND
ND
(Rao et al., 2016)
Novokinin (RPLKPW)
Modified β-conglycinin α’ subunit 1x Novokinin in 3 homologous sites of the core domain flanked by trypsin and/or chymotrypsin cleavage sites
5.2 (42h)2
ND
-25 (4h)2
102
(Matoba et al., 2001a)
Modified β-conglycinin α’ subunit 1x Novokinin in 3 homologous sites of the core domain plus 1x Novokinin in a homologous site of the extension domain; flanked by gastrointestinal proteases cleavage sites
ND
ND
-18 (4h)2
2.52
(Onishi et al., 2004)
VY
Modified amarantin acidic subunit 4xVY in the third variable region (VR3); C-terminal His tag AACM.3
60 (3h)2
2.02
ND
ND
(Luna-Suárez et al., 2010)
Modified amarantin acidic subunit 4xVY in the fourth variable region (VR4); C-terminal His tag AACM.4
560 (6h)2
ND
ND
ND
(Morales-Camacho et al., 2016)
VY and RIPP
Modified amarantin acidic subunit 4xVY in VR3 and 1xRIPP in VR4; C-terminal His tag AACM3.4
50 (3h)2
1.412
ND
ND
(Castro-Martínez et al., 2012)
VY and IPP
Modified amarantin acidic subunit 4xVY in VR3 and 1xIPP in VR4; C-terminal His tag AMC3
ND
56402
-58 (4.5h)2
1002
(Medina-Godoy et al., 2013)