Whole transgenic plant
|
Rice (seeds)
|
Novokinin (RPLKPW)
|
Fusion with glutelin B1 (pGluB1-HRP)
2x Novokinin in 2 HVR of GluB1 acidic subunit plus 1x Novokinin in the C
terminal VR of GluB1 basic subunit
|
oral administration of crude glutelin B1 fraction
|
-28±7 (4h)
|
0.03
|
(Yang et al., 2006)
|
|
|
|
|
oral administration of unpolished pulverized GluB1-HRP rice
seeds |
Not significant |
1.00 |
|
|
|
|
Fusion with glutelin A2 and C (pGluA2-HRP/GluC-HRP)
2x Novokinin in 2 HVR of GluA2 and GluC acidic subunits
|
oral administration of unpolished pulverized GluA2-HRP/GluC-HRP rice
seeds
|
-15.6±4.8 (2h)
|
1.00
|
|
|
|
|
Tandem structure
10x Novokinin + KDEL; 18x Novokinin + KDEL
|
oral administration of transgenic seeds containing 18x novokinin
|
-15.6±1.1 (4h)
|
1.00
|
(Wakasa et al., 2011)
|
|
Soybean (seeds)
|
Novokinin (RPLKPW)
|
Fusion with β-conglycinin α’ subunit
1x Novokinin in 4 sites
|
oral administration of β-conglycinin fraction
|
-16.0±1.5 (8h)
|
0.15
|
(Yamada et al., 2008)
|
|
|
|
|
oral administration of defatted flour |
-16.4±4.3 (6h) |
0.25 |
|
|
|
|
Fusion with β-conglycinin α’ subunit
4x Novokinin
|
ND
|
ND
|
ND
|
(Nishizawa et al., 2008)
|
|
Tomato (fruits)
|
VY
|
Fusion with acidic subunit of amarantin
4x VY + KDEL
|
ND
|
ND
|
ND
|
(Germán-Báez et al., 2014)
|
Cell suspension cultures
|
Tobacco (calli)
|
VY
|
Fusion with acidic subunit of amarantin
4x VY + KDEL
|
ND
|
ND
|
ND
|
(Santos-Ballardo et al., 2013)
|
Transplastomic
|
C. reinhardtii (chloroplasts)
|
VLPVP
|
Tandem structure
6x VLPVP
|
Oral administration of freeze-dried biomass
|
-20 (6h)
|
0.30
|
(Ochoa-Mendez et al., 2016)
|
|
|
GLDIQK
VAGTWY LDAQSAPLRLLQK
VLVLDTDYK CMENSA ALPMHIR
|
Multivariate chimeric peptide
|
ND
|
ND
|
ND
|
(Campos-Quevedo et al., 2013)
|