aT7 promoter was used in all reports, except in (Lv et al., 2003) (T5 promoter), (Liu et al., 2007) and (Rao et al., 2009) (tac promoter) and (Renye and Somkuti, 2015) (nisA promoter)
bThe presented values correspond to the higher peptide expression levels obtained. Peptide expression was induced with IPTG, excepting (Renye and Somkuti, 2015), where nisin was used instead.
cPeptides were orally administered, excepting (Medina-Godoy et al., 2013), where recombinant protein hydrolysates were administered by gastric gavage.
ND- Not determined
NR- Not recovered
1mg of peptide per g of wet weight cells
2 Value for the chimeric protein
3 Value for Rabbit lung ACE
4 Value for Human plasma ACE
5 Value for Human umbilical vein endothelial cells (HUVECs) ACE
6Value for the peptide multimer
7BP1-3 complete amino acid sequences (the bioactive sequences are underlined): BP1, QTQSLVYPFPGPI PNS; BP2, LPQNIPPLTQTPV VVP; and BP3, EDELQDKIHPF AQTQS. Correspond to fragments 57–66, 73–82, and 47–52 of bovine β-casein (A2 allelic variant), respectively.
Table 3. ACEI peptides expressed as recombinant constructs in plant and algae platforms. ‘Anti-HT’: antihypertensive activity (mmHg); ‘Dose’: dose of peptides in g per Kg of body weight. HVR, hypervariable region; VR, variable region.