Scaffold preparation
Uniform scaffold samples from all scaffold modifications were prepared using a rectangular punch (0.5 x 0.2 cm2) and autoclaved. On the day of the experiment, scaffolds were transferred to 96-well plates and 100 µl pancreatic islets of Langerhans cell culture complete growth media (Celprogen) was added to each well. Scaffolds were incubated for 2 hours at 37° C. Culture media was removed before seeding the cells.