MTT (3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyltetrazolium bromide) Viable Cell Assay
After seeding primary pancreatic cells and hiPSC-derived pancreatic cells on scaffolds, 1, 3, 5 and 10 days after seeding, the viable cell density on each scaffold was assessed by MTT assay (Invitrogen). Six replicates were performed for each scaffold modification. Following the manufacturer’s instructions, scaffold culture media of cells grown on scaffolds were changed to fresh media and 10 µl reconstituted MTT reagent was added to each well of the 96-well plate. Scaffolds were incubated overnight. The next day, MTT solution was aspirated and 100 µl solubilization solution was added to scaffolds. Solubilized MTT solutions were transferred to clean 96-well plates and solution absorbance was measured at 570 nm on a Tecan infinite 200 microplate reader.