Scaffold Modification
Wicking cellulose scaffolds were manufactured by a proprietary ”slit and
expanded” chemical/mechanical process causing formation of a honeycomb
structure with simultaneous heightened airflow and liquid wicking.
Amine-modified cellulose scaffolds were manufactured by derivatizing the
unmodified cellulose with sodium (meta) periodate followed by a solution
of ethylenediamine dihydrochloride, borax and sodium borohydride.
Hydroxyl-modified scaffolds were prepared by immersing unmodified
scaffolds in 1% NaOH solution for 1 hour at 100° C, followed by 3
washes in each distilled water and phosphate buffered saline (PBS) and
air drying overnight. All three scaffolds, unmodified (U),
amine-modified (A) and NaOH-modified (N) were also immersed in 1%
gelatin solution for 2 hours in room temperature, followed by air drying
overnight to yield UG, AG and NG scaffolds, respectively.