Scaffold preparation
Uniform scaffold samples from all scaffold modifications were prepared
using a rectangular punch (0.5 x 0.2 cm2) and
autoclaved. On the day of the experiment, scaffolds were transferred to
96-well plates and 100 µl pancreatic islets of Langerhans cell culture
complete growth media (Celprogen) was added to each well. Scaffolds were
incubated for 2 hours at 37° C. Culture media was removed before seeding
the cells.