MTT (3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyltetrazolium
bromide) Viable Cell Assay
After seeding primary pancreatic cells and hiPSC-derived pancreatic
cells on scaffolds, 1, 3, 5 and 10 days after seeding, the viable cell
density on each scaffold was assessed by MTT assay (Invitrogen). Six
replicates were performed for each scaffold modification. Following the
manufacturer’s instructions, scaffold culture media of cells grown on
scaffolds were changed to fresh media and 10 µl reconstituted MTT
reagent was added to each well of the 96-well plate. Scaffolds were
incubated overnight. The next day, MTT solution was aspirated and 100 µl
solubilization solution was added to scaffolds. Solubilized MTT
solutions were transferred to clean 96-well plates and solution
absorbance was measured at 570 nm on a Tecan infinite 200 microplate
reader.