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Contrasting physiological responses of Chlamydomonas reinhardtii to fibrous and layered clay minerals
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  • Zhongquan Jiang,
  • Sijia Wu,
  • Zhongyang Wang,
  • Zhonghua Zhao,
  • Lu Zhang,
  • Zhen Li,
  • YING GE
Zhongquan Jiang
Nanjing Agricultural University
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Sijia Wu
Nanjing Agricultural University
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Zhongyang Wang
Nanjing Agricultural University
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Zhonghua Zhao
Nanjing Institute of Geography and Limnology Chinese Academy of Sciences
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Lu Zhang
Nanjing Institute of Geography and Limnology Chinese Academy of Sciences
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Zhen Li
Nanjing Agricultural University
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YING GE
Nanjing Agricultural University College of Resources and Environmental Sciences

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Abstract

Microalgae interact with mineral particles in aqueous environment, yet how clay minerals affect physiological processes in algal cells remain unexplored. In this study, we compared the effects of palygorskite (Pal) and montmorillonite (Mt), which respectively represent fibrous and layered clay minerals, on the physiological processes of Chlamydomonas reinhardtii. It was observed that C. reinhardtii responded differently to the treatments of Pal and Mt. The Pal particles bound tightly to and even inserted cells, resulting in a significantly decrease of cell numbers from 27.35 to 21.02*107 mL-1. However, Mt was only loosely attached to the cell surface. The photosynthesis in the algal cells was greatly inhibited by Pal, with rETRmax significantly reduced from 103.80 to 56.67 μmol electrons m-2s-1 and downregulation of IF2CP, psbH and OHP1, which were key genes involved in photosynthesis. In addition, Pal reduced the quantities of proteins and polysaccharides in extracellular polymeric substances (EPS) and the P uptake by C. reinhardtii when the P level in the culture was 3.15 mg/L. However, no significant changes were found regarding the above EPS components or the amount of P in algal cells upon the addition of Mt. Together, the impacts of fibrous Pal on C. reinhardtii was more profound than those of layered Mt.
25 Oct 2023Submitted to Journal of Basic Microbiology
27 Oct 2023Submission Checks Completed
27 Oct 2023Assigned to Editor
27 Oct 2023Review(s) Completed, Editorial Evaluation Pending
03 Nov 2023Reviewer(s) Assigned